油菜黑胫病菌Leptosphaeria biglobosa生物学特性研究

摘要

This paper focused on biological characteristics of Leptosphaeria biglobosa, the causal agent of blackleg disease on Chinese oilseed rape, as well as the effects of temperature, pH, illumination, nitrogen source and carbon source on mycelial growth and sporulation of the pathogen. Results showed that L. biglobosa could conduct mycelial growth and sporulation at the temperature between 5℃ and 35℃ with the optimum temperature at 25℃. The pathogen produced spores under pH4 to pH10, the optimum at pH7. Alternate light and darkness was favorable to mycelial growth and sporulation, while continuous light inhibited the production of mycelia and conidia in particular. The optimal carbon source and nitrogen sources were soluble starch and beef extract respectively. The lethal temperature for conidia of L. biglobosa was 52℃.%为了解我国油菜黑胫病病原菌(Leptosphaeria biglobosa)的生物学特性,在实验室里研究了温度、pH值、光照、碳源及氮源等条件对该病菌营养生长及分生孢子产生的影响.结果表明:病原菌在5℃～35℃均可进行营养生长并产孢,最适生长和产孢温度为25℃；在pH4～10的范围内该菌均能生长并产孢,在pH7的条件下生长最快,产孢最多.光暗交替有利于菌丝生长和产孢,而持续光照不利于菌丝生长,尤其不利于产孢.最适的碳源、氮源分别是可溶性淀粉、牛肉膏.分生孢子的致死温度为52℃.