橘林油脂酵母发酵条件的初步研究

摘要

The oil - producing conditions of Lipomyces kononenkoae were optimized on flask fermentation level by the single - factor experiment. The highest lipid yield was obtained with ammonium sulfate and glucose as nitrogen source and carbon source, respectively. The optimum culture conditions for temperature, inoculation, initial pH value and C/N ratio were 28t, 10% , 7.0 and 99, respectively. Batch culture result showed that the biomass, lipid content and lipid yield of Lipomyces kononenkoae could reach 7.01g/L, 15.91% , 1. 12g/L respectively after optimization. The results of flask fed - batch cultures indicated the dry biomass and lipid yield of Lipomyces kononenkoae could further reach 24. 13g/L and 3. 06g/L respectively.%以橘林油脂酵母(Lipomyces kononenkoae CICC1714)为实验材料,通过设计单因素实验,确定了摇瓶发酵培养的最佳产油条件为:氮源为硫酸铵,碳源为葡萄糖,培养温度28℃,接种量10％,初始pH值为7.0,最适碳氮比为99.优化后批次发酵的生物量为7.01g/L,油脂含量15.91％,油脂产量1.12g/L.经半连续发酵后最终的生物量为24.13g/L,油脂产量为3.06g/L.