甘蓝型油菜隐性核不育上位基因抑制作用专一性研究

摘要

将甘蓝型油菜隐性上位互作核不育系9012A的临时保持系12 - 148 - 204 - F14TAM、204TAM分别与双隐性核不育系5A杂交,研究隐性上位抑制基因rfrf对5A中不育基因ms1 ms1 ms2ms2的抑制作用.结果表明rfrf基因对ms1 ms1 ms2ms2的不育性表达没有抑制作用,而对9012A中不育基因ms3 ms3 ms4 ms4具有专一抑制作用.%Inhibiting effect of rfrf on mslmslms2ms2 genes was evaluated using two crosses of 12 -148 -204 - F14TAM (temporary maintainer line)×5 A and 204TAM (temporary maintainer line)×5A respectively. The rfrf gene was from recessive epistatic genie male sterile line 9012A (Brassica napus L. ) , and the mslmslms2ms2 genes were from double recessive genie male sterile line 5A' (Brassica napus L. ). Result showed that rfrf had no inhibiting effect on msmsms2ms2, but had specific inhibition effect on ms3ms3ms4ms4 sterile genes from 9012A.