湖南省油菜品种的种传链格孢菌检测

摘要

2012年在湖南醴陵市油菜黑霉病果上经镜检和病种分离检出链格孢（Alternaria spp．），其后检测了36个品种的种子带菌情况，华湘油12号带菌率最高（51％）。镜检结果发现链格孢菌有2种，rDNA-ITS序列分析表明，它们分别是芸薹生链格孢（A．brassicicola）和芸薹链格孢（A．brassicae）；未检测到萝卜链格孢（A．raphani）。36个品种中芸薹生链格孢分离频率最高的是华湘油12号（66％），且全为芸薹生链格孢，而芸薹链格孢以常油杂61为最高（15％），且全为芸薹链格孢。禾盛油868种子上未检测到链格孢菌。%Alternaria spp.was detected on rapeseed siliques with black mold collected from Liling city of Hu-nan Province under microscope in 2012.Among 36 tested commercial rapeseed cultivars,Huaxiangyou 12 had the highest Alternaria contamination frequency (up to 5 1%)by microscope on seed detection.Isolates were proved to be A.brassicicola and A.brassicae respectively by rDNA-ITS sequence analysis.Huaxiangyou 12 had the highest i-solation rate (66%)and only carried A.brassicicola.Changyouza 6 1 had the highest A.brassicae isolation frequen-cy (15%).No A.raphani was isolated.Heshengyou 868 had neither of the Alternaria spp.