转甲状腺素蛋白对视网膜微血管内皮细胞生物学行为的影响

摘要

Objective To explore the effects of transthyretin (TTR)on biological behavior of retinal microvascular epithelial cell (RMVEC).Methods RMVEC was cultured in medium with 0 μmol/L and 4 μmol/L TTR.The proliferation,migration and healing abilities (0,24,48 hours)of RMVEC with different concentrations of TTR were measured by methyl thiazol tetrazolium (MTT)assay,transwell assay and scarification test.Results MTT assay shows that RMVEC with the concentrations of 4 μmol/L TTR [absorbance (A)value=0.1 7±0.02]glows faster than with the concentrations of 0 μmol/L TTR (A value=0.40± 0.03 ),the difference was statistically significant (t = 1 5.47,P = 0.000 1).The transwell assay shows RMVEC with the concentration of 4 μmol/L TTR [(140 ± 7 )cells]migrants faster than RMVEC with the concentration of 0 μmol/L TTR [(227±14)cells],the difference was statistically significant (t=5.44,P =0.000 6).The scarification test shows that the RMVEC with the concentration of 4 μmol/L TTR [(134.4±45.4)μm]heals faster than the RMVEC with the concentration of 0 μmol/L TTR [(330.0 ± 23.1)μm],the difference was statistically significant (t = 8.25,P < 0.01 ).The cells in 48 hours and 4 μmol/L group were healed completely,but not healed in 0 μmol/L group.Conclusion TTR can promote the proliferation,migration and healing abilities of RMVEC.%目的：观察转甲状腺素蛋白(TTR)对视网膜微血管内皮细胞(RMVEC)生物学行为的影响。方法培养的 RMVEC 依照 TTR 浓度不同分为0(初始浓度)、4μmol/L 组。培养48 h 后,噻唑蓝(MTT)比色法、细胞迁移实验分别检测不同浓度 TTR 对 RMVEC 增生、迁移的影响。细胞划痕实验检测培养0(初始时间)、24、48 h 时细胞损伤愈合情况。结果MMT 比色法检测结果显示,初始浓度组、4μmol/L 组吸光度[A,旧称光密度(OD)]值分别为0.17±0.02、0.40±0.03。两组 A 值比较,差异有统计学意义(t =15.47,P =0.0001)。细胞迁移实验结果显示,初始浓度组、4μmol/L 组 RMVEC 向外迁移数分别为(140±7)、(227±14)个。两组间 RMVEC 向外迁移数比较,差异有统计学意义(t=5.44,P =0.0006)。细胞划痕实验结果显示,初始时间各组细胞划痕距离大致相等；24 h,初始浓度组、4μmol/L 组细胞划痕愈合距离分别为(134.4±45.4)、(330.0±23.1)μm。两组细胞划痕愈合距离比较,差异有统计学意义(t =8.25,P <0.01)。48 h,4μmol/L 组完全愈合,初始浓度组未愈合。结论TTR 对 RMVEC 增生、迁移和损伤愈合有明显促进作用。