S100B 蛋白表达与早发型及晚发型子痫前期发病的关系

摘要

目的 通过检测S100B蛋白在早发型及晚发型子痫前期胎盘组织滋养细胞中的表达,探讨S100B蛋白表达与早发型及晚发型子痫前期发病的关系.方法 选择2010年10月-2011年9月在青岛市市立医院产科以剖宫产术分娩的子痫前期产妇60例,其中早发型子痫前期产妇30例(早发型组,发病孕周＜ 34周),晚发型子痫前期产妇30例(晚发型组,发病孕周≥34周),随机选择同期健康足月妊娠(因骨盆异常、臀位、巨大儿、社会因素等原因)行剖宫产术分娩的产妇30例为健康对照组.采用逆转录(RT) -PCR技术以及免疫组化方法分别检测各组产妇胎盘滋养细胞中S100BmRNA及蛋白的表达.结果 (1)S100B mRNA在各组胎盘滋养细胞中均有表达,其中在早发型组为0.73±0.11,晚发型组为0.64±0.10,健康对照组为0.58±0.08；其早发型组与晚发型组及健康对照组比较,差异有统计学意义(P＜0.05)；晚发型组与健康对照组比较,差异无显著意义(P＞0.05).(2)S100B蛋白在各组胎盘滋养细胞中均有表达,主要表达于胎盘滋养细胞的细胞膜、细胞质内,呈棕黄色或棕褐色颗粒.S100B蛋白在早发型组的阳性表达率为100％( 30/30),在晚发型组的阳性表达率为70％(21/30),健康对照组的阳性表达率为63％ (19/30),早发型组与晚发型组及健康对照组比较,差异有统计学意义(P＜0.05)；晚发型组与健康对照组比较,差异无统计学意义(P＞0.05).结论 早发型与晚发型子痫前期可能存在不同的发病机制,S100B蛋白参与了早发型子痫前期的发病.%Objective To investigate the relationship of S100B protein expression and the pathogenesis of early-onset and late-onset preeclampsia.Methods Sixty patients with preeclampsia who received caesarean section at Qingdao Municipal Hospital from October 2010 to September 2011 were enrolled in this study.Thirty cases were early-onset preeclampsia( referred as early-onset preeclampsia group,＜ 34 weeks),and the other 30 cases were late-onset preeclampsia (referred as late-onset preeclampsia group,≥34 weeks).Thirty women who received caesarean section because of pelvic structural deformities,breech presentation,macrosomia and social factors were included as the control group.The expression of S100B mRNA in the placenta was detected by reverse transcription ( RT)-PCR.The expression of S100B protein in the placenta was detected by immunohistochemistry.Results ( 1 ) S100B mRNA was expressed in the trophoblasts of preeclampsia and control groups.The expression of S100B mRNA in early-onset preeclampsia group (0.73 ±0.11 ) was significantly higher than the control group (0.58 ±0.08) and lateonset preeclampsia group (0.64 ±0.10,P ＜0.05 ).There was no significant difference between late-onset preeclampsia group and the control group ( P ＞ 0.05 ).(2) S100B protein was expressed in the plasma membrane and cytoplasm of the trophoblasts,correlated positively with the brownish yellow and brown particles inside the cells.It was expressed in all the three groups.Immunohistochemistry revealed that the expression of S100B protein in the placenta of early-onset preeclampsia group was 100％ (30/30),significantly higher than those of late-onset preeclampsia group and the control group,in which the positive rate were 70％ (21/30) and 63％ (19/30) respectively (P ＜0.05).There was no difference between late onset preeclampsia group and the control group (P ＞0.05).Conclusion Early-onset and late-onset preeclampsia may have different etiology and pathogenesis.S100B may be a factor in the pathogenesis of early-onset preeclampsia.