中药提取物CTE对MPTP所致帕金森病小鼠模型的神经保护作用

摘要

目的:研究中药提取物CTE对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)所致帕金森病小鼠模型是否具有神经保护作用.方法:首先建立MPTP所致帕金森病小鼠模型,连续4 d给予MPTP(30 mg·kg-1,qd,ip).用高低剂量(50和100 mg·kg-1,qd,ig)中药提取物CTE预处理MPTP所致帕金森模型小鼠.之后进行行为学检测,包括一般行为学检测和滚筒实验.最后,用HPLC-EC法测定纹状体中多巴胺(DA)及其代谢产物二羟苯乙酸(DOPAC)和高香草酸(HVA)含量.结果:在MPTP所致的帕金森病小鼠模型,中药提取物CTE能显著改善小鼠的行为能力;且能显著提高小鼠纹状体内多巴胺含量.结论:中药提取物CTE对MPTP所致帕金森病小鼠模型有神经保护作用.%Objective: To investigate the neuroprotective effect of CTE, an extract from traditional Chinese medicine, in a mouse model of Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Methods: We induced Parkinson's disease model by MPTP (30 mg·kg-1, qd, ig). After pretreatment with high and low doses (50 and 100 mg·kg-1 , qd, ig) of CTE, behavioral changes were tested after MPTP induction. The content of dopamine and its metabolites ( DOPAC and HVA) were determined by HPLC-EC. Results: In the mouse model of MPTP-induced Parkinson's disease, CTE significantly improved the behavior dysfunctions, and significantly increased levels of dopamine in the striatum. Conclusion: The traditional Chinese medicine extract CTE has neuroprotective effect in the mouse model of MPTP-induced Parkinson's disease.