Objective: To establish an ion-pair reversed-phase chromatographic method for the determination of vitamin B1, B2 and B6 in compound vitamin tablets. Methods: The contents of vitamin B1, B2 and B6 were simultaneously determined by ion-pair reversed-phase high performance liquid chromatography. Results: The linear ranges of vitamin B1, B2 and B6 were 6.3~14.7 μg· mL-1 ( r = 0. 999 60) , 7. 2 ~ 16. 8 μg·mL-1 ( r = 0. 999 1 ) and 6.3~14. 7 μg·mL-1(r =0. 999 2) with the average recoveries of 100. 08% (RSD=0.84%, n=9), 100.35% ( RSD = 1. 26% , n = 9 ) and 99. 41 % ( RSD = 1.10%, n = 9 ) , respectively. Conclusion: The method is simple, rapid, accurate and reliable, and can be used for the assay and quality control of compound vitamin tablets.%目的:建立反相离子对色谱法测定复方维生素片中维生素B1、维生素B2、维生素B6含量的方法.方法:以已烷磺酸钠作为离子对试剂,采用Diamonsil C18(250 mm×4.6mm,5μm)柱,同时测定复方维生素片中维生素B1、B2和B6的含量.结果:维生素B1、B2和B6分别在6.3 ～14.7 μg·mL-1(r=0.9998),7.2 ～ 16.8 μg·mL-1(r =0.9995),6.3 ～14.7 μg·mL-1(r=0.9996)范围内线性关系良好,平均回收率分别为100.08％(RSD =0.84％,n=9),100.35％(RSD=1.26％,n=9),99.41％(RSD=1.10％,n=9).结论:本方法快速、简便、准确,分离效果好,辅料无干扰,可用于该类制剂中维生素B1,B2和B6的含量测定及质量控制研究.
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