创伤性脑损伤后大鼠海马区糖皮质激素受体mRNA的表达

摘要

Objective To explore the expression of glucocorticoid receptor (GR) mRNA in rat hippocampus and its effect on cognitive function after traumatic brain injury (TBI). Methods The rat TBI model by lateral acceleration rotation of head was established. GR mRNA levels were detected by reverse transcription polymerase chain reaction (RT-PCR), and cognitive function was evaluated by Morris water maze.Results Expression of GR mRNA in rat hippocampus remained in low level from 4 d to 7 d after TBI. Morris water maze test showed significant impairment of cognitive functions in rats. Conclusion Down-regulation of GR mRNA in hippocampus induces the cognitive dysfunction in rats.%目的 研究创伤性脑损伤(TBI)后大鼠海马区糖皮质激素受体(GR)mRNA表达的变化及其对大鼠认知功能的影响.方法 建立大鼠头颅侧向旋转加速脑创伤模型,应用逆转录酶-聚合酶链式反应(RT-PCR)和Morris水迷宫检测伤后大鼠海马区GR mRNA的表达与学习记忆功能的关系.结果 伤后4～7 d大鼠海马区GR持续低表达;Morris水迷宫检测伤后大鼠出现认知功能障碍.结论 TBI大鼠海马区GR mRNA的降低影响大鼠认知功能.