NF-KB p65及环氧化酶-2在实验性变态反应性脑脊髓炎中的表达及其作用机制

摘要

Objective To study the expression of nuclear factor-kB (NF-KB) and COX-2 and their relationship in experimental allergic encephalomyelitis (EAE), and investigate their role in the pathogenesis of EAE. Methods All rabbits were classified three groups in according to making models by different immunogens: group A is immuned without immunogen, group B is immuned with R-SCH, group C is immunod with C-SCH. Immunofluorescence and immunohistochemistry were performed for morphological observations of the brain tissues in all animals, and then the activity of NF-KBp65 was detected using enzyme-linked immunosorbent assay. Results The expressions of NF-KBp65 and COX-2 were observed in brain issues with EAE. Significant differences were found in the number of cells positive for NF-KBp65 and COX-2 between EAE groups and the control group. NF-KBp65 and COX-2 expressions were associated with the symptoms of EAE. In addition, significantly increased activity ofNF-kBp65 was noted in EAE, which is immuned with R-SCH. Conclusion NF-KB and COX-2 are expressed in rabbits with EAE and play important roles in the pathogenesis of EAE.%目的 研究核转录因子NF-kB p65及环氧化酶-2(COX-2)在实验性变态反应性脑脊髓炎(EAE)中的表达,探讨其参与EAE的作用机制. 方法 健康新西兰兔30只根据采用不同免疫原制作模型的方式分为三组:A组,正常对照组(未注射免疫原)10只;B组,新西兰兔脊髓匀浆(R-SCH)EAE模型组,10只;C组,牛脑匀浆(C-SCH)EAE模型组,10只.比较每组动物临床病理特点;分别采用免疫荧光、免疫组化对其进行形态观察,应用ELISA法对NF-KBp65进行活性测定.结果 B、C组均可见NF-kB p65及COX-2明显增加,阳性细胞百分率与A组比较.差异有统计学意义(P<0.05);其中B组NF-kB p65活性明显增高.结论 在EAE模型中存在NF-kBp65及COX-2表达,它们均在EAE发病过程中发挥了重要作用.