氟哌啶醇致僵直大鼠皮质高电压纺锤波节律及面部肌电特征

摘要

目的 研究氟哌啶醇对成年大鼠在自由活动状态下大脑皮质高电压纺锤波( high-voltage spindles,HVSs)及相应面部肌电活动的影响.方法 通过在体多通道电生理记录技术结合视频录像,对可自由活动的大鼠进行皮质脑电和面部肌电长程记录,观察氟哌啶醇致僵直大鼠皮质HVSs节律数量及相应面部活动及肌电的变化.结果 在清醒静止状态下,正常大鼠自发出现HVSs节律,面部可见触须震颤,经腹腔注射氟哌啶醇1.0mg/kg和3.0 mg/kg后,大鼠呈现僵直状态,HVSs节律数量增加,面部抽搐活动增多,波及范围扩大,强度增加,肌电显示节律性活动增多、持续时间延长.结论 氟哌啶醇作为多巴胺D2受体拮抗剂可增强HVSs节律及面部抽搐,提示多巴胺D2受体参与HVSs节律的调节.%Objective To study the effects of haloperidol on neocortical high-voltage spindles (HVSs) and accompanied facial muscular activities in freely moving adult rats. Methods Continuous video, neocortical electroencephalogram (EEG) and facial electromyography (EMG) were recorded simultaneously for more than six hours in freely moving rats. The number of HVSs and the facial muscular activities were analyzed after administration of saline or haloperidol. Results Spontaneous HVSs and whisker twitching were observed in normal rats during behavioral waking-immobility. In the haloperidol-induced catalepsy rats (intraperitoneal injection of 1.0 mg/kg and 3.0 mg/kg haloperidol, respectively), the number of HVSs and companied facial rhythmic muscular activities increased; tremor intensity was enhanced in eyes, ears and even in the dorsal of neck without gross body movement, the duration of rhythmic muscular activities was prolonged. Conclusions Haloperidol, the dopamine D2 receptors antagonist, could enhance HVSs and facial twitching, indicating that dopamine D2 receptors maybe contribute to the regulation of the HVSs, and the abnormal HVSs may be the underlying electrophysiological mechanism of extrapyramidal symptoms ( EPS) induced by antipsychotic treatments.