荷瘤荧光裸小鼠高压氧实验的通风笼屏障系统研制

摘要

Objective To develop the hyperbaric oxygen barrier system which was smtable for experiments in tumor-bearing fluorescence nude mice and met the NASA100 level high efficiency filter air standards.Methods An air inlet and outlet composite pipe frame connected with the air intake was designed.The composite pipe frame air inlet and outlet ducts were connected with the air inlet and outlet of the independent ventilation cage (IVC).Inside the filter was a NASA100 level high efficiency filter membrane.Results The device was called the HBO-IVC,which was harbored in the 0.2 MPa oxygen-rich HBO chamber.With the help of pressure potential,the oxygen-rich air was filtered twice with the high efficiency filter of the HBO-IVC and the membrane of the independent ventilation cage.As a consequence,attached dust and pathogens could be thoroughly filtered,thus ensuring the biological features of tumor-bearing fluorescent nude mice in the pathogen-free environment.Conclusions The HBO-IVC was suitable for experimental researches of HBO intervention in tumor-bearing fluorescence nude mice raised in the pathogen-free environment.%目的 研制适合荷瘤荧光裸小鼠行高压氧实验、达到NASA100级高效过滤空气的高压氧屏障装置(HBO-IVC).方法 设计1个有进出风管组合的管道框架和与之连接的进气过滤装置.进出风管与小鼠独立通气笼的进出风口衔接,进气过滤装置内部设有NASA100级的高效过滤器.结果 设计的HBO-IVC置于0.2 MPa高压氧舱内的富氧气体中,利用压力差将富氧气体经过HBO-IVC进气装置的高效过滤器和独立通气笼过滤膜2次过滤,能将灰尘及附在灰尘上的外来病原体过滤掉,较好地保持了必须在无特殊病原体环境中饲养的荷瘤荧光裸小鼠的生物学特征稳定.结论 HBO-IVC适合在无特殊病原体环境中饲养的荷瘤裸小鼠行高压氧干预的实验研究.