白头翁汤正丁醇提取物对小鼠外阴阴道念珠菌病的治疗作用研究

摘要

目的 探讨白头翁汤正丁醇提取物(Butyl alcohol extract of BaiTouWeng,BAEB)对小鼠外阴阴道念珠菌病(vulvovaginal candidiasis,VVC)的治疗作用.方法 雌性昆明种小鼠72只,随机分为正常组、模型组、白头翁汤正丁醇提取物高剂量、中剂量、低剂量与氟康唑组,皮下注射雌二醇致小鼠假发情期,再阴道接种白念珠菌2×106 CFU/mL,药物治疗7d.采用涂布法检测VVC小鼠的阴道真菌载荷量;HE染色法观察阴道组织的病理学改变;ELISA法检测小鼠阴道组织的IL-1β、IL-18的含量;qRT-PCR法检测小鼠阴道组织中NLRP3、ASC、caspase-1的mRNA表达.结果 与正常组相比,模型组小鼠阴道黏膜层完全破坏,大量炎症细胞浸润;与模型组比较,BAEB组黏膜呈现不同程度的改善.与正常组相比较,模型组小鼠阴道组织IL-1β、IL-18含量升高,真菌载量明显升高,均具有统计学意义(P＜0.001).阴道组织中TLR2、TLR4、NLRP3、ASC、caspase-1的mRNA含量分别上调,差异均有统计学意义(P＜0.001).与模型组相比较,BAEB治疗组IL-1β、IL-18含量均降低,差异均有统计学意义(P＜0.001).结论 白头翁汤正丁醇提取物对小鼠外阴阴道念珠菌病具有治疗作用.%Objective To study the effects of Butyl alcohol extract of BaiTouWeng decoction (BAEB) on vulvovaginal candidiasis (VVC) in murine model.Methods Seventy-two female mice were randomly divided into normal group,model group,BAEB high-dose group,BAEB middle-dose group,BAEB low-dose group,and fluconazole group.The mice were firstly injected with estradiol to induce oestrum,and then intravaginally administrated with 2 × 106 CFU/mL Candida albicans.After 7-day treatment,The fungal load (CFU/mL) was assessed from vaginal lavage fluid,the pathological changes of vaginal tissues were observed by HE staining.The levels of IL-1β and IL-18 in vaginal tissues were detected by ELISA.The mRNA expressions of NLRP3,ASC,caspase-1 were analyzed by qRT-PCR.Results Compared with the normal group,the vaginal mucosa of the model group was completely destroyed,and the infiltrations of plenty of inflammatory cells were observed.Compared with the model group,BAEB groups showed improvements to varying degrees.The levels of IL-1β and IL-18 in the model group were significantly higher than those in the normal group (P ＜ 0.001).The mRNA expressions of TLR2,TLR4,NL-RP3,ASC and caspase-1 were up-regulated significantly (P＜0.001).The levels of IL-1β and IL-18 in the BAEB groups were much lower than those in the control group (P＜0.001).Conclusion The BAEB could be used to treat the vulvovaginal candidiasis in mice.