目的 评估CFW (Caleofluor white)荧光染色法诊断甲真菌病的阳性率、准确性和效率.方法 临床怀疑甲真菌病患者的病甲,同时进行10％KOH溶液湿片法镜检、真菌培养和CFW荧光染色法镜检,计算各自真菌检出阳性率,并统计CFW法与KOH镜检法或真菌培养的差异,比较KOH与CFW镜下特点.结果 共入组100例拟诊为甲真菌病患者.CFW法镜检阳性率64％,KOH法镜检阳性率63％,真菌培养阳性率31％.CFW法与KOH法镜检阳性率比较差异无统计学意义(P＞0.05);CFW法镜检与真菌培养阳性率比较差异有统计学意义(P＜0.01).但CFW制片比KOH更加简单、快速,镜下显示的真菌更清晰易辨.结论 CFW荧光染色法是一种方便、快速、准确性较高的诊断方法,可以提高甲真菌病诊断的准确性和效率.%Objective Evaluate the positive rate,accuracy and efficiency of CFW (Calcofluor white) fluorescent staining for the diagnosis of suspected onychomycosis.Methods Outpatients suspected to have onychomycosis were recruited.Each patient was subjected to three methods (CFW fluorescent staining,KOH microscopy and fungal culture) together to confirm the diagnosis.Then we compared the positive rates and the statistically significant differences across the three methods.Moreover,the images under KOH and CFW were also compared.Results One hundred patients with 100 affected nails were recruited.Among them,the positive rates of the CFW,KOH,and culture were 64 ％,63 ％,and 31％,respectively.According to the McNemar test,CFW had a higher percentage of positive rate than culture testing (P＜0.01),but it was not statistically significant different from KOH (P＞0.05).Compared with KOH,the preparation and examination time were faster and easier,and the fungal structures under CFW were clearer,more obvious,and more easily detected.Conclusions CFW fluorescent staining was a simple,fast,convenient,accurate,and time-efficient method.It could potentially increase the accuracy and efficiency of the diagnosis of onychomycosis and help to avoid misdiagnosis.
展开▼
