Objective To observe the effects of exogenous testosterone replacement and androgen receptor block agent administration on the expression of senescence-associated-β-galactosidase(SA-P-Gal), and to determine the relationship between androgen and myocardial senescence. Methods Forty male Wistar rats were randomly divided into castrated group, testosterone group (castrated + physiological dose of testosterone), flutamide group (castrated + physiological dose of testosterone+flutamide) and sham group, with 10 in each group. After 8 weeks, blood testosterone concentration was measured; myocardium tissues were examined for histomorphology by HE staining; activity of SA-β-Gal was determined immunohistochemically. Results Compared with sham operation group, testosterone concentration was almost three times higher than that in flutamide group (P<0.01). The activity of SA-β-Gal was obviously enhanced in castrated group than in sham group[(2.00 ± 0.86) vs (1.15 ±0.67), P<0.01]. Compared with castrated group, the activity of SA-β-Gal was significantly weakened in testosterone group[(0.90 ± 0.79), P < 0.01]. Compared with testosterone group, the activity of SA-β-Gal was enhanced in flutamide group[(2.05 ± 0.76), P < 0.01]. There was no statistical difference in the activity of SA-β-Gal between testosterone group and sham group ( P=0.309 ) . Conclusions Androgen deficiency may influence the activity of SA-β-Gal in the heart of male rats. It suggests that myocardial senescence is possibly associated with androgen levels.%目的 观察补充外源性睾酮及给予雄激素受体阻断剂后对衰老相关p-半乳糖苷酶( SA-β-Gal)的影响,探讨雄激素与心脏衰老的关系.方法 雄性Wistar大鼠40只随机分为去势组(10只)、补充睾酮组(去势+生理剂量睾酮组,10只)、氟他胺组(去势+生理剂量睾酮+氟他胺组,10只)、假手术组(10只).干预8周后测定各组血浆睾酮浓度,取心肌组织用HE染色观察心肌组织学变化；组织化学染色观察心肌组织细胞SA-β-Gal的活性.结果 较假手术组睾酮浓度,氟他胺组升高近3倍(P＜0.01)；与假手术组比较,去势组SA-p-Gal活性明显增强[(2.0±0.9)vs(1.2±0.7),P＜0.01]；与去势组比较,生理剂量补充睾酮组SA-β-Gal活性(0.9±0.8)明显减弱(P＜0.01)；与单纯补充生理剂量睾酮组比较,氟他胺组SA- β-Gal活性(2.1±0.8)反而增强(P＜0.01)；补充生理剂量睾酮组与假手术组SA-β-Gal的活性相比差异无统计学意义(P=0.309).结论 雄激素缺乏可能影响雄性大鼠心肌组织中SA-β-Gal的活性,提示心脏老化可能与雄激素水平相关.
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