压疮护理SD大鼠模型的建立

摘要

目的 建立适于研究压疮预防护理的动物模型.方法 健康雄性SD大鼠30只,随机分 为6组,每组5只,麻醉后使其仰卧于设计好的简易加压装置,以大鼠后肢关节为压迫点,每组的压力大小分别为70,64,58,53,48,44mm Hg,持续作用2 h后,观察受压部位皮肤的外观改变,并应用寇氏法(K(a)rbar氏法)计算使半数动物受压部位皮肤发红的压强.结果 各组均未出现肉眼可见的皮肤破损等严重变化,但受压局部皮肤肉眼观察可见发红现象.在此实验条件下,使半数动物皮肤发红的压强大小为55.08 mm Hg.结论 在此装置下,建立这个模型最合适的压强应为55.08 mm Hg.以此压强建立的SD大鼠动物模型方法简单易行,可应用于预防压疮护理的动物实验研究.%Objective To establish an SD rats animal model for preventive nursing of pressure ulcer.Methods 30 healthy male SD rats were randomly and averagely divided into 6 groups, which were put in simple compression devices supinely after anesthesia, with their hind limb joints regarded as oppressed points.The pressure of each group was: 70, 64, 58, 53, 48 and 44mmHg, respectively. By observing skin color change after two hours of compression and then calculating LD50, the pressure that made half animals' compression skin red was obtained. Results No group had visible skin damages and other serious changes, but compression local skin redness was found. Under the experimental conditions, the pressure of the skin redness among half of the animals was 55.08 mmHg. Conclusions In this model, the most appropriate pressure was 55.08 mmHg. The SD rats pressure ulcer animal model is feasible and could be applied to animal studies on preventive nursing of pressure ulcer.