骨髓间充质干细胞治疗肌萎缩侧索硬化症的护理

摘要

目的 观察骨髓间充质干细胞(MSC)治疗肌萎缩侧索硬化症(ALS)的临床效果,探讨护理方法.方法 在无菌条件下采集患者骨髓液,密度梯度离心法分离,获取单个核细胞层,接种于培养瓶中,加入培养液,在5%二氧化碳细胞培养箱培养3d,微生物检测安全后静脉输注给患者.结果 治疗后能延迟肌萎缩侧索硬化症患者的病情进展,有效8例,无效1例.结论 MSC治疗肌萎缩侧索硬化症的临床效果有效.%Objective To observe the therapeutic effects of bone marrow-derived mesenehymal stem cells(BMSCs)in the treatment of amyotrophie lateral sclerosis(ALS).Methods To collect bone marrow in asepsis conditions.Mononuclear cells were collected by gradient centrifugation,cultured in growth medium and 5%carbon dioxide for 3 days.BMSCs were analyzed by flow cytometry with a FACScan(Becton Dickinson). All BMSCs were transplanted into patients intra venous.Results After BMSCs treatment.8 patients with ALS had obvious clinical effects,1 patients had no response to treatment.Conclusions BMSCs treatment for ALS has obvious clinical effect.