DNA含量和倍体类型与食管鳞状细胞癌淋巴结转移的关系

摘要

Objective To investigate the correlation of DNA content, DNA ploidy status and lymph node metastasis of esophageal squamous cell carcinoma(ESCC). Methods DNA analysis was carried out respectively by flow cytometry in the specimens from 167 patients with resectable ESCC. DNA index (DI) and ploidy status was measured respectively. Then analyze the correlation of DI and DNA ploidy status with lymph node metastasis of ESCC. Results ①Heteroploidy rate in ESCC was 81.44%. Even in early stage of ESCC, the diversity of DNA index and ploidy status could be detected; ②DNA index was higher in group with lymph node metastasis than that without lymph node metastasis;③Comparing with ESCC with DNA diploidy, ESCC with DNA heteroploidy showed higher lymph nodes metastasis rate and lymph node metastasis ratio. Lymph node metastasis occurred even in early stage of ESCC with DNA heteroploidy. Conclusion Esophageal squamous cell carcinoma with higher DNA content or DNA heteroploidy more likely metastasizes to lymph node.%目的：探讨DNA含量和倍体类型与食管鳞状细胞癌淋巴结转移的关系。方法采用流式细胞技术对手术切除的167例食管鳞癌患者新鲜标本进行DNA分析，计算DNA指数并判断倍体类型，分析DNA含量和倍体类型与食管鳞癌淋巴结转移情况的关系。结果①食管鳞癌患者异倍体出现率为81.44%，食管鳞癌在早期就已出现了DNA含量和倍体类型的改变；②有淋巴结转移组食管鳞癌DNA含量明显高于无淋巴结转移组食管鳞癌(t=2.53；P=0.012)；③异倍体食管鳞癌淋巴结转移率和淋巴结转移度明显高于二倍体(χ2=4.06；P=0.044和χ2=7.23；P=0.007)，且早期即可出现淋巴结转移。结论 DNA含量高的食管鳞癌和异倍体食管鳞癌更容易发生淋巴结。