Objective:To investigate the effects of berberine on the pathological damages of gastric nerve plex-us,cardiac and mesenteric blood vessels in the early diabetic rats.Method:1 5 SD rats were inj ected streptozotocin (STZ 65mg/kg),12 rats were divided into two groups randomly,one group was model group (n=6),another group was treated with berberine (n=6).After two weeks of the diabetic model was successfully made,berberine solvent was administrated by gavage for two weeks,and same amount of double-distilled water was given a gavage to the rats in model group simultaneously.The same batch of rats fed a normal diet as control group (n=6 ).After two weeks of treated with berberine,the colour of fur,investigated body weight and blood glucose of all rats were observed.And then tissues were taken,paraffin sections were made.The number of neuron nissl bodies which from the submucosal neurons of gastric body and fundus,and the cardiac and vascular pathological changes were deteated by the method of tolyidine blue and eosin dye.Results:Compared with the control rats,the hair and tail color were much darker,body weight was reduced (P<0.01),and the level of blood glucose was increased significantly (P<0.01)in the diabetic rats.There were no apparently differences in the body weight (P>0.05)and the level of blood glucose (P>0.05)between barbering administrated and diabetic rats,but for berberine group,the dark color of tail were dropped down and the hair were more luster.In the diabetic rats,the number of neuron nissl bodies in nerve plexus of gastric body and fundus were decreased or disappeared,while the cardiac and mesenteric blood vessels e-merged obvious morphological changes and blood stasis.And in berberine treated rats,the populations of neuron nissl bodies were increased,the morphological changes and blood stasis were ameliorated.Conclusion:Berberine a-meliorates the damage of gastric nerve,cardiac and mesenteric vascular in early diabetic rats.%目的：：观察小檗碱对早期糖尿病大鼠胃肠神经元、肠系膜和心脏微血管损伤的改善作用。方法：清洁级SD 大鼠15只,采用腹腔注射链脲佐菌素(STZ,65 mg/kg)制备糖尿病模型,12只成模大鼠随机分为模型组(n=6)和小檗碱组(n=6)。成模2周后小檗碱组以小檗碱溶液(200mg/kg)灌胃2周,模型组灌胃等量双蒸水。同批次普通饲料喂养的大鼠作为对照组(n=6)。给药2周后观察各组大鼠毛色、体重和血糖,并取各组大鼠胃壁、肠系膜和心脏制备组织病理切片。通过甲苯胺蓝-伊红染色观察其胃体、胃底黏膜下神经元尼氏体数量及肠系膜微血管和心肌微血管的组织病理学变化,比较各组各指标的差异。结果：实验28天后,与对照组大鼠相比,模型组大鼠饮水、排尿量增加,毛色暗哑,尾部变黑,体重较对照组明显降低,血糖水平较对照组明显升高(P 均<0.01)。小檗碱组较模型组大鼠毛色有光泽,尾部黑色现象有所减退,但体重及血糖水平改善均不明显(P 均>0.05)。模型组大鼠胃体、胃底黏膜下神元尼氏体数量较对照组减少甚至消失,肠系膜微血管皱缩且瘀血严重,心肌微血管肌层变薄。小檗碱组胃壁神经丛尼氏体数量较模型组增多,肠系膜微血管变形、瘀血及心肌微血管情况均较模型组有所改善。结论：小檗碱对早期糖尿病大鼠胃壁神经及肠系膜和心脏微血管损伤有一定保护作用。
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