Objective To study the function of gene 0955 in Streptococcus suis type 2 98HAH33. Methods Growth condition of wild-type, mutant and complemented strains of Streptococcus suis type 2 98HAH33 was compared at different stages. Differences in adhesion ability to host cells and anti-phagocyto-sis among these strains were compared by using bacterial adhesion test and analyzing their survival rates in blood. Mouse and piglet models were used to evaluate their virulence. Results The growth of the mutant and the complemented strains was slightly slower than that of the wild type strains in logarithmic growth phase, but no significant difference was found in plateau phase. Bacterial adhesion test showed that gene 0955 might encode a new adhesion factor of Streptococcus suis type 2 98HAH33. Blood bactericidal test sug-gested that gene 0955 was not associated with anti-phygocytosis. Animal experiments showed that gene 0955 might be a novel virulence gene of Streptococcus suis type 2 98HAH33. Conclusion Gene 0955 might en-code a novel adhesion factor and virulence factor of Streptococcus suis type 2 98HAH33.%目的 对猪链球菌2型98HAH33基因0955进行功能研究.方法 比较野生株、突变株和回复株在不同时期的生长情况,细菌黏附和血中存活比较它们对宿主黏附和抗吞噬能力的差异,小鼠和仔猪模型比较它们在毒力方面的差异.结果 突变株和回复株在对数生长期生长略慢于野生株,但在平台期生长情况一致;细菌黏附证明基因0955可能是猪链球菌2型98HAH33的一个新黏附因子;血中存活证明基因0955和抗吞噬无关;小鼠和仔猪实验证明基因0955 可能是猪链球菌2 型98HAH33的一个新毒力因子.结论 猪链球菌2型98HAH33基因0955可能是新鉴定的黏附因子和毒力因子.
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