川穹嗪改善Aβ1-42致痴呆模型小鼠学习记忆功能实验研究#

摘要

目的：探讨川穹嗪对于Aβ1-42致痴呆模型小鼠学习记忆功能的影响，并对其机制进行初步研究。方法：通过避暗实验、跳台实验检测小鼠学习记忆功能；通过胞内钙影像测定胞内钙浓度变化。结果：避暗实验和跳台实验均表明，高中低剂量组的川穹嗪与模型组相比，能显著增加潜伏期，减少错误次数(P<0.05)；胞内钙影像结果表明，在有钙或无钙外液两种情况下，川穹嗪均降低海马神经元胞内钙离子浓度。结论：川穹嗪可显著改善Aβ1-42致痴呆模型小鼠学习记忆功能，其机制可能与川穹嗪降低胞内钙离子浓度，从而调节神经元兴奋性，突触可塑性和信号传递有关。%Objectives:To discuss tetramethylpyrazine (TMP)’s influence on learning and memorial function of Aβ1-42 caused AD model mouse and its potential mechanism. Methods:Tested mouse’s learning and memorial function through step-through test and step-down test;Measured calcium concentration through intracellular calcium imaging. Results:Behavior test showed that compared to model group, TMP can increase the latent period and reduce error times significantly(P<0.05);calcium image results showed that TMP can reduce calcium concentration of hippocampal neurons in the extracellular solution with calcium or without calcium. Conclusion:TMP can improve learning and memorial functions of Aβ1-42 caused AD mouse significantly. The mechanism may due to TMP can reduce intracellular calcium ion concentration, therefore regulate neuronal excitability, neuronal plasticity and signal transmission.