目的 探讨不同辐照模式聚焦超声诱导人胰腺癌细胞凋亡的最佳辐照方案.方法 对人胰腺癌细胞系PaTu 8988t细胞悬液进行聚焦超声辐照:第1组为空白对照组,第2、3组采用脉冲辐照模式,第4、5组采用连续辐照模式,各组辐照剂量通过改变辐照时间来改变.以流式细胞仪检测辐照后24 h肿瘤细胞凋亡率及细胞死亡率.结果 超声辐照后各组的细胞悬液最高温度分别为28.00℃、(42.20±2.17)℃、(50.80±0.84)℃、(55.80±2.17)℃、(65.20±3.11)℃,各组间差异均有统计学意义(P均<0.05).各辐照组的细胞凋亡率为(0.56±0.15)%、(1.28±0.16)%、(1.84±0.29)%、(5.74±1.15)%、(2.00±0.84)%,细胞死亡率为(3.28±0.59)%、(4.60±0.47)%、(8.64±2.69)%、(41.12±15.91)%、(49.70±8.02)%,各组间差异均有统计学意义(P均<0.05).结论 聚焦超声辐照剂量在接近热凝固温度阈值时,采用连续辐照模式可获得较高的细胞凋亡率及细胞死亡率.%Objective To explore the optimal radiation pattern of f'ocused ultrasound induced pancreatic carcionoma cell apoptosis . Methods The human pancreatic carcinoma cell line PaTu 8988t suspension was radiated by focused ultrasound using 5 dose groups with dif'f'erent radiation time parameters and patterns . Pulsed radiation pattern was used in groups 2 and 3 , while continuous radiation pattern was used in group 4 and 5 , group 1 was taken as the controls . The ratio of focused ultrasound induced cell apoptosis and cell death was tested with flow cytometer 24 h after ultrasound radiation . Results The maximum cell suspension temperature of 5 dose groups was 28. 00℃ . (42. 20±2. 17 )℃ . (50. 80 ± 0. 84 )℃ .(55. 80±2. 17 )℃ and (65. 20±3. 11 )℃ , respectively (all P＜ 0. 05) . The cell apoptosis rate was (0. 56±0. 15 )％ . (1. 28±0. 16 )％ . (1. 84±0. 29 )％ . (5. 74±1. 15 )％ and (2. 00±0. 84 )％ , respectively f'or 5 dose groups , and the cell death rate was (3. 28±0. 59 )％ . (4. 60±0. 47 )％ . (8. 64±2. 69 )％ . ( 41. 12±15. 91 )％ and (49. 70±8. 02 )％ , respectively (all P＜ 0. 05 ) . Conclusion Using threshold thermal exposures of focused ultrasound radiation with continuous radiation pattern is able to acquire high ratio of cell apoptosis .
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