HDGF在手术切除非小细胞肺癌中的异常表达及其在预测预后中的意义

摘要

Background and objective Our previous studies revealed that hepatoma-derived growth factor (HD GF) is highly expressed in non-small cell lung cancer (NSCLC) cells, playing important roles in promoting NSCLC cells growth and invasion.The aim of this study is to detect the expression of HDGF in 158 cases of surgically resected NSCLC and evaluate its clinical significance.Methods Immunohistochemical SP method was used to detect the expression of HDGF in 158 NSCLC tissues and 12 normal control lung tissues.Survival analysis was further conducted.Results HDGF was found significantly highly expressed in 158 NSCLC tissues compared with normal control lung tissues (P＜0.001.The 5-year survival rate was 38.2％ in HD GF high expression cases, compared with 63.1％ in HD GF low expression cases, the difference was statistically significant (P=0.009).Linear correlation analysis discovered a significantly negative correlation between HDGF expression and the survival time (r=-0.183, P=0.022).COX proportion hazard model analysis revealed that pathological stages and HDGF expression were independent prognostic factors for this group of 158 resected NSCLC cases.Conclusion HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs.It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs.%背景与目的 在前期研究中发现肝癌衍生生长因子(hepatoma-derived growth factor,HDGF)在A549、H226等非小细胞肺癌( non-small cell lung cancer,NSCLC)细胞中明显高表达,在促进NSCLC细胞侵袭、生长、迁移过程中起重要作用.本研究旨在进一步检测HDGF在NSCLC组织标本中的表达情况,探讨其临床意义.方法 应用SP法.检测158例手术切除NSCLC及12例正常对照肺组织中HDGF蛋白表达情况,进行生存分析、预后判定.结果 与12例正常对照肺组织比较,HDGF蛋白在158例NSCLC中明显高表达(P<0.001).158例NSCLC中,HDGF高表达组(78例,占49.4%)的5年生存率为38.2%,明显低于HDGF低表达组(80例,占50.6%)的5年生存率63.1%(P=0.009).直线相关分析表明HDGF表达水平与术后生存时间呈负相关(r=0.183,P=0.022).多因素生存分析表明术后病理分期和HDGF表达水平是手术切除NSCLC预后判定的独立因素.结论 HDGF在NSCLC中呈现高表达;HDGF高表达代表预后不良,HDGF可以作为手术切除NSCLC预后判定的新的分子标志物.