Background and objective There exsits intimate relationship between infection with chlamydia pneumoniae (Cpn) and lung cancer incidence. But few studies have been reported about radiation-induced pulmonary lesion in lung cancer patients infected with Cpn. The aim of this study is to explore the correlation between cytokines related to radiation-induced pulmonary lesion and Cpn IgG positive in lung cancer patients. Methods A total of 69 patients with lung cancer received chest radiotherapy. Blood samples were collected and frozen before radiotherapy (pre-RT), middle radiotherapy (mid-RT) and after radiotherapy (post-RT). Cpn IgG and levels of IL-1β, SP-A, TGF-β, and TNF-α were measured by enzymelinked immunosorbent assay (ELISA). Results In the total of 69 patients, 21 patients were Cpn IgG positive, 48 patients negative. The positive rate was 30.43％. In mid-RT concentration of IL-1 β in Cpn IgG positive and negative group were (35.82 ± 1 0.09) ng/L and (30.01 ±6.46) ng/L, with statistically significant difference (P＜0.05). Pre-RT and post-RT concentrations of IL- 1 β in Cpn IgG positive and negative group had no statistically significant difference. Mid-RT concentrations of SP-A in Cpn IgG positive group and negative group were (641.78±106.81) ng/L and (100.86±61.4) ng/L respectively, with statistically significant difference (P＜0.05). Post-RT concentration of SP-A in Cpn IgG positive and negative group were (657.47± 115.19) ng/L and (93.23±47.15) ng/L respectively, with statistically significant difference (P＜0.05). Concentrations of TNF-α in Cpn IgG positive and negative group had no statistically significant difference. Concentrations of TGF-β in Cpn IgG positive group were (710.67±358.16) pg/mL in pre-RT, (1,002.06±542.16) pg/mL in mid-RT, (2,125.16±1,522.29) pg/mL, in post-RT;those in negative group were (867.77±412.48) pg/mL, (914.05±425.70) pg/mL, (1,073.36±896.01) pg/mL. Concentration of TGF-β in post-RT between Cpn IgG positive and negative group had statistically significant difference (P＜0.05). Conclusion Cpn IgG positive in lung cancer patients influenced levels of IL-1β, SP-A, TGF-β during chest radiotherapy. This might aggravate radiation-induced pulmonary lesion.%背景与目的 肺炎衣原体感染与肺癌有密切关系,但肺炎衣原体感染对肺癌放射性肺损伤的影响尚未见报道.本研究旨在探讨肺癌患者肺炎衣原体(chlamydia pneumoniae,Cpn)IgG阳性对放射性肺损伤相关细胞因子的影响.方法 观察有病理诊断、初次胸部放疗肺癌患者69例,分别于放疗前、放疗中和放疗后采血冻存,采用酶联免疫吸附法检测血液中Cpn的IgG以及IL-1β、SP-A、TGF-β、TNF-α的含量.结果 69例肺癌患者中Cpn IgG阳性21例,阴性48例,阳性率为30.43%.Cpn IgG阳性组与阴性组放疗中IL-1β浓度分别为(35.82±10.09)ng/L和(30.01±6.46)ng/L,两组之间有差异无统计学意义(P<0.05),放疗前和放疗后无明显差异.Cpn IgG阳性组与阴性组放疗前SP-A的浓度差异无统计学意义,放疗中分别为(641.78±106.81)ng/L和(100.86±61.4)ng/L,有统计学差异(P<0.05),放疗后浓度分别(657.47±115.19)ng/L和(93.23±47.15)ng/L,也有统计学差异(P<0.05).Cpn IgG阳性组与阴性组放疗前、放疗中、放疗后TNF-α浓度两组之间均无统计学差异.Cpn IgG阳性组放疗前、放疗中、放疗后TGF-β1的浓度分别(710.67±358.16)pg/mL、(1,002.06±542.16)pg/mL和(2,125.16±1,522.29)pg/mL,阴性组分别为(867.77±412.48)pg/mL、(914.05±425.70)pg/mL和(1,073.36±896.01)pg/mL,两组之间比较放疗后TGF-β1浓度有统计学差异(P<0.05).结论 肺癌患者Cpn IgG阳性一定程度上提高了放射性肺损伤相关细胞因子SP-A、TGF-β1、IL-1β的水平,提示可能加重肺癌患者的放射性肺损伤.
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