磷脂酰肌醇蛋白多糖-3在肝细胞肝癌的表达及其与术后复发的相关性

摘要

目的 探讨磷脂酰肌醇蛋白多糖-3(GPC-3)mRNA在肝细胞癌(HCC) 癌组织及癌旁组织的表达及其与术后复发的相关性.方法 用逆转录聚合酶链反应测定60例HCC癌组织及癌旁组织中GPC-3 mRNA的表达.PCR产物经凝胶电泳,比较HCC组织及癌旁组织中GPC-3与β-actin条带的灰度值之比,半定量分析GPC-3 mRNA的表达水平,分析术后3年不易复发HCC患者的癌组织GPC-3 mRNA的表达水平.结果 GPC-3 mRNA在HCC癌组织中高度表达,阳性率为68.3%,癌旁组织中低表达或未见表达,术后3年内未复发的HCC患者癌组织中GPC-3 mRNA呈现低表达水平,GPC-3 mRNA表达与肿瘤大小、肿瘤数目、有无包膜、门静脉癌栓、HBsAg及AFP水平无明显相关.结论 GPC-3 mRNA在HCC癌组织中的表达明显高于癌旁组织,可能是HCC的一种新的潜在的肿瘤特异标志物;癌组织中GPC-3 mRNA低表达的HCC患者不易复发,可能是判断HCC进展和评价预后的一个潜在重要指标.%Objective 　To investigate the expressions and recurrence after surgery of GPC-3 mRNA in hepatocellular carcinoma （HCC ） and paratumor tissues .Methods 　RT-PCR was used to detect the expression of GPC-3 mRNA in samples of HCC tissue from 60 patients .PCR products were tested by gel electrophoresis ，the expressions of GPC -3 gene in HCC and paratumor tissues were semiquantified by calculating the ratio of the gray value of GPC -3 bands and those of β-actin bands respectively ，to investigate GPC-3 mRNA expression levels of HCC patients after 3 years without recurrence .Results　The expression of GPC-3 mRNA in the HCC tissues was significantly higher ，expression rate was 68 .3％ ，lowor no expression was detectable in paratumor tissue .GPC-3 mRNA of expression showed low levels of HCC tissue after 3 years without recurrence .The expression of GPC-3 mRNA was not with tumor size ，tumor number ，whether the envelope ，tumor thrombus in portalvein and the level of HBsAg or AFP in serum .Conclusion 　GPC-3 mRNA in the tumors was significantly higher than that in the paratumor tissues ，it might be a new genetic marker of HCC ；GPC-3 mRNA expression low levels may without recurrence of HCC patients ，it might be a potential value in judging progression and evaluation of prognosis.