深圳市妇女宫颈癌组织中人乳头瘤病毒16型E6、E7致癌基因的序列分析

摘要

Objective To investigate the coding sequences of HPV1 6 E6/E7 gene extracted from the cervical cancer tissues from patients in Shenzhen.Methods The HPV1 6 E6/E7 gene was extracted from a HPV1 6 positive cervical cancer patient that had been living in Shenzhen for 1 7 years.The gene was amplified by PCR and cloned;the recombi-nant plasmid was constructed;and the DNA Sequence was restricted by restriction endonuclease.Results The HPV1 6 E6/E7 gene was successfully cloned and the sequencing analysis showed that the cloned HPV1 6 E6/E7 gene is identical to the German standard strain documented in GenBank.Conclusion The coding of HPV1 6 E6/E7 gene extracted from cervical cancer tissues from female patient in Shenzhen is identical to the German standard strain.%目的：检测深圳市宫颈癌患者癌组织中 HPV16型 E6、E7致癌基因的序列。方法采用 PCR 技术扩增1例在深圳生活17年宫颈癌 HPV16型阳性患者组织中 HPV16 E6、E7基因，对其进行克隆、构建重组质粒并进行核酸限制性内切酶鉴定及DNA测序分析。结果成功地克隆了 HPV16 E6、E7基因，测序分析表明克隆的 HPV16 E6、E7基因与 GenBank收录的德国标准株相比完全一致。结论深圳市妇女宫颈癌组织中 HPV16型 E6、E7致癌基因的序列与德国标准株相同。