荧光定量 PCR 检测 miRNA-155在过敏性皮肤病中的应用研究

摘要

目的：建立荧光定量 PCR 技术检测外周血 miRNA-155相对含量检测方法，研究过敏性皮肤病患者外周血miRNA-155表达量与正常对照组的差异。方法利用 miRNA 分离纯化盒提取外周血血浆中的 miRNA，逆转录合成cDNA 后，采用 SYBR Green 荧光染料法进行定量 PCR 检测40例荨麻疹患者、30例皮肤湿疹患者和30例健康对照组外周血中 miRNA-155的表达含量。结果荨麻疹患者外周血中的 miRNA-155的表达水平明显高于正常对照组（（P ＜0.01），也高于皮肤湿疹患者（P ＜0．05）；皮肤湿疹患者与正常对照组比较 miRNA-155的表达水平无明显差异（P ＞0.05）。结论荧光定量 PCR 技术检测外周血 miRNA-155相对含量有助于荨麻疹的诊断，为进一步研究荨麻疹的发病机制奠定实验基础。%Objective To use Fluorescence Quantitative PCR technique to detect the relative content of the miRNA-155 in peripheral blood,and study the differences of the expression quantity of the miRNA-155 in peripheral blood be-tween allergic dermatosis patients and normal controls.Methods Use miRNA separation and purification box to extract miRNA from peripheral blood plasma,after reverse transcription of cDNA,use SYBR Green method to carry on the quantitative PCR test to detect the expression quantity of the miRNA-155 in peripheral blood among 40 cases of urticar-ia patients,30 cases of eczema patients and 30 cases of healthy controls.Results The expression level of miRNA-155 in peripheral blood of urticaria patients was significantly higher than that of the control group (P <0.01)and was also higher than that of eczema patients (P <0.05).There is no significant difference of the miRNA-155 expression level between eczema patients and normal control group (P >0.05).Conclusion The detection of the relative content of the miRNA-155 in peripheral blood with Fluorescence Quantitative PCR technique is conductive to the diagnosis of urticari-a,laying an experimental foundation for further study of the pathogenesis of urticaria.