乌司他丁注射液改善急性肺损伤兔循环内皮祖细胞成血管能力及对PKB／eNOS／NO通路的影响

摘要

O bjective To observe the ability of ang iogenesis in acute lung injury circulating endo thelial progenitor cels after uli-nasta tin injected and ang iogenesis of PKB /eNOS /NO pathw ay affected.M ethods The cels in vitro w ere divided into 5 g roups :The contro l group , acute lung injury , ulinastatin treatm ent group , ulinastatin treatm ent + LY294002 group , ulinastatin treatm ent + L NAME groups .Ang iogenicability ,PKB , eNOS pro tein ,NO concentration w ere detected.Results The ability of ang iogenesis in ulinas-ta tin treatm ent group w as higher than that in acute lung injury groups ( P < 0.05) .The PKB , eNOS pro tein expression increased in ulinastatin treatm ent g roup ,and there w as differences betw een ulinastatin treatm ent g roup and acute lung injury groups ( P < 0.05) . NO concentra tion w as increased in ulinastatin treatm ent group , and there w as differences betw een ulinastatin treatm ent g roup and a-cute lung injury g roups ( P < 0.05) .Conclusion U linastatin injection could enhance the ability of ang iogenicability on circulating en-do thelial progenitor cels through PKB / eNOS /NO pathw ay.%目的：观察乌司他丁注射液对急性肺损伤循环内皮祖细胞血管生成能力及对 PKB /eNOS /NO 通路的影响。方法体外细胞实验共分为5组：正常对照组、急性肺损伤组、乌司他丁治疗组、乌司他丁＋ LY294002组，乌司他丁＋ L NAME组分别进行体外皮祖细胞成血管能力检测；PKB、eNOS 蛋白检测；NO 浓度检测。结果乌司他丁治疗组较急性肺损伤组体外成血管数目增加， PKB、eNOS蛋白表达上升，NO 浓度上升，差异有统计学意义（ P ＜0．05）。结论乌司他丁注射液通过 PKB /eNOS /NO 通路，提高循环内皮祖细胞成血管能力。