麝香保心丸对实验大鼠血管钙化的作用

摘要

目的：在大鼠血管钙化模型上,探讨麝香保心丸对血管钙化的影响及其可能作用机制。方法实验动物随机分正常组、钙化组及钙化+麝香保心丸组,每组6只。钙化组采用维生素 D3(3×105 U/kg 1次,肌肉注射)和尼古丁(25 mg/kg溶于花生油中,早、晚各灌胃1次)诱导大鼠血管钙化模型,钙化+麝香保心丸组于造模后第2天开始,每天麝香保心丸灌胃[14.0 mg/(kg· d)],用Von Kossa染色检测血管钙化程度,用钙离子测试盒、碱性磷酸酶(ALP)试剂盒测定大鼠主动脉钙含量和 ALP活性,用放射免疫法检测大鼠血浆单核细胞趋化蛋白1(MCP 1)含量,用免疫组织化学法检测血管组织 MCP 1受体表达。结果维生素 D3和尼古丁能够诱导典型大鼠血管钙化模型,Von Kossa染色可见血管钙化模型大鼠主动脉有大量黑色颗粒沉淀,钙化组血管钙含量、ALP活性高于正常组(P<0.01),同时,血浆MCP浓度、血管组织MCP 1及其受体表达明显上调(P<0.01)；用麝香保心丸干预后,血管钙化程度减轻(P<0.01)。血浆MCP 1及其受体的表达下调,差异有统计学意义。结论麝香保心丸可抑制血管钙化,并且提示可能通过下调 MCP 1表达和 ALP活性抑制血管钙化的发生和发展过程。%Objective To investigate the effect and possible mechanism of Shexiang Baoxin Pil (SBP)in aorta of vascular calcification model rats.Methods Seven week old male Sprague Dawley (SD)rats were randomly divided into normal group,calcification group and calcification plus SBP group (al n= 6).On the first day in calcification and calcification plus SBP group,vascular calcifi-cation was induced by a single dose intramuscular injection of vitamin D3 (300 000 U/kg)in the morning plus two doses of nicotine (25 mg/kg dissolved in peanut oil )gavage in the morning and in evening.The next day after calcification induction,rats in SBP group were gavaged with SBP[14 mg/(kg· d)]for four weeks.Vascular calcification was confirmed by Von Kossa staining.Calcium content and alkaline phosphatases (ALP)activity were detected by calcium assay kit and ALP detection kit respectively.The contents of plasma monocyte chemotactic protein 1 (MCP 1)were determined by radioimmunoassay,and the expressions of correspond-ing receptors were determined by immunohistochemistry.Results Von Kossa staining showed that there were mass black granules deposited in aortic wal of the vascular calcified rats.Calcium content and ALP activity in calcified group were increased significantly than those in the control group (P<0.01).Meanwhile,MCP 1 contents in plasma and corresponding receptor level in calcified group were up regulated significantly compared with those in the control group(P<0.01).In addition,SBP diet could reduce the degree of vascular calcium contents,ALP activity and MCP 1 content.Conclusion SBP significantly protects the aorta against cal-cification partly through reducing MCP 1 content and ALP activity.