目的:探讨左归丸对5/6肾大部切除模型并肾性骨病大鼠骨代谢的影响.方法:SPF级Wistar大鼠进行5/6肾大部切除并给予高磷饮食诱导肾性骨病模型.造模大鼠分为模型组、左归丸组、骨化三醇组,并设假手术组.左归丸与骨化三醇治疗4周、12周时,检测血肌酐(Scr)、尿素氮(BUN)、血清碱性磷酸酶(ALP)、血清全段甲状旁腺激素(iPTH)、血钙(Ca2+)、血磷(P3-)；药物干预12周麻醉处死大鼠,双能X线测量大鼠股骨骨密度(BMD).结果:12周时,模型组大鼠出现了Scr、BUN、ALP、iPTH、磷升高,血Ca2+下降.左归丸能明显降低Scr、BUN水平,与模型组比较差异有统计学意义(P＜0.01,P＜0.05)；与骨化三醇组比较差异有统计学意义(P＜0.01,P＜0.05).左归丸组大鼠ALP、iPTH、血磷水平与模型组比较明显下降(P＜0.05),血钙水平明显升高(P＜0.01),左归丸组大鼠骨密度与模型组比较明显改善(P＜0.01).结论:左归丸通过调节钙磷代谢及参与成骨细胞代谢,对iPTH有直接的抑制作用,能改善肾性骨病的骨营养不良.%Objective: To investigate of the beneficial effects of Zuoguiwan on the bone metabolism in 5/6 nephrectomy - induced renal osteodystrophy(ROD) rat. Methods:24 rats were 5/6 nephrectomized and fed with high dietary phosphorus, the other 8 rats were dealt with pseudo - operation. The nephrectomized rats were divided into model group, Zuoguiwan group, and Calcitriol group (each n=8). The other 8 rats were normal group. After 4 weeks and 12 weeks treatment, blood Scr, BUN, ALP, iPTH, Ca and P were measured; the rats were sacrificed at the end of 12 weeks treatment. The femur bone mineral density (BMD) was measured by dual energy X-ray. Results:In the model group, blood Scr, BUN, ALP, iPTH and P levels were significantly increased, and blood Ca levels were decreased. The levels of Scr and BUN were significantly lower in Zuoguiwan group than that in model group ( P<0.01,P<0.05) and that in Calcitriol group(P <0. 01 ,P <0. 05 ) . Blood levels of ALP、PTH、P were significantly lower and Ca was significantly higher in Zuoguiwan group than that in model group ( P < 0. 05 ). The BMD was significantly higher in Zuoguiwan group than that in model group( P < 0. 01) . Conclusion: Conclusions: Zuoguiwan can improve renal osteodystrophy. The treatment effect perhaps has relation with decreaseing iPTH level, adjusting metabolism of bone tissue.
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