从糖肾宁对糖尿病肾病大鼠糖脂代谢的影响研究其肾脏保护作用

摘要

目的：探讨糖肾宁对糖尿病肾病大鼠糖脂代谢的影响及其肾脏保护作用的机制。方法：雄性SD大鼠40只，采用腹腔内注射福氏完全佐剂、链脲佐菌素及高脂饲料喂养的方法建立糖尿病肾病大鼠模型。按24h尿微量白蛋白（24hU-Alb）测定值随机分为模型组（M组）、糖肾宁组（T组），格列喹酮组（G组）和正常组（N组）各8只，8周后，检测各组大鼠空腹血糖、空腹胰岛素（INS）、糖化血红蛋白（HbA1c）、三酰甘油（TG），胆固醇（TC）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、24hU-Alb及肾功能。结果：M组空腹血糖、TC、LDL显著升高，INS、HDL明显下降。治疗8周后T组INS明显上升，TC、LDL显著下降、HDL明显升高，24hU-Alb减少，肾功能显著改善，且明显优于G组。结论：格列喹酮和糖肾宁均可不同程度影响血糖水平，糖肾宁还可有效调节脂代谢。糖肾宁可能是通过调节肾组织糖代谢及血脂代谢，延缓了肾小球硬化及肾间质纤维化进程，发挥了独立于降糖作用之外的降蛋白及肾功能保护作用。%Objective:To study the effect and mechanism of Tangshenning Recipe on kidney protection from glucose and lipid metabolism in rats with diabetic nepropathy. Methods:50 male SD rats were randomly divided into high fat diet for 3 weeks,was injected intraperitoneally with STZ and CFA at the same time. After 2 weeks,24h U-Alb was measured. According to 24h U-Alb,the 24 SD rats were randomly divided into diabetic model group(group M,n = 8),Tangshenning group(group T,n = 8)and gliquidone group(group G,n = 8). After 8 weeks,the level of fasting blood - glucose,fasting insulin(INS),glycated hemoglobins (HbA1c),triglyceride(TG),cholesterol(TC),high - density lipoprotein(HDL),low density lipoprotein(LDL),creatinine(Cr), urea nitrogen(BUN)and 24h U-Alb was measured. Results:The levels of blood glucose,TC in rats of group M were obviously higher than those of group N. On the other side,the levels of INS and HDL were obviously lower than those of group N. After 8 weeks treatment the levels of INS and HDL were ascended,but TC declined markedly. Corresponding to these,the excretion of 24h U-Alb,Cr and BUN significant improvement of group T. Conclusion:Gliquidone and Tangshenning can regulate blood glucose in differ-ent degree,and the latter can regulate lipid metabolism also. Tangshenning give play to reducing the proteinuria and protecting renal function independent of glucose decrease through the regulation of nephridial tissue glucose metabolism and lipid metabolism in serum.