参地补肾胶囊对肾小球硬化大鼠MMP-9、TIMP-1及其受体mRNA表达的影响∗

摘要

Objective:To observe the effects of shendi bushen capsule on the m RNA expressions of MMP-9, TIMP-1 and its receptor in rats with glomerulosclerosis ( GS) . Methods:GS rat model was established by unilateral nephrectomy and adriamy-cin injection. SD rats were randomly divided into blank control group, model group, shendi bushen capsule group, benazepril group and suhuang xiezhuo pill group. Serum creatinine ( Scr) , blood urea nitrogen ( BUN) and plasma albumin ( Alb) were detected. Im-munohistochemical method was used to detect the expressions of MMP-9 and TIMP-1, and the ratio of MMP-9/TIMP-1 was cal-culated. RT-PCR was used to detect the expression level of TIMP-1 receptor m RNA. Results:Compared with the model group, shendi bushen capsule could obviously inhibit TIMP-1 and its receptor m RNA expressions (P<0. 01), increase MMP-9 expres-sion (P<0. 01), and enhance the ratio of MMP-9/TIMP-1. Conclusion:Shendi bushen capsule can inhibit the expressions of TIMP-1 and its receptor m RNA, improve the renal function so that it may retard the development of glomerular sclerosis.%目的：：观察参地补肾胶囊对肾小球硬化大鼠基质金属蛋白酶(MMP-9)、基质金属蛋白酶抑制物(TIMP-1)及其受体mRNA表达的影响,探讨其抗肾小球硬化的机制。方法：采用单侧肾脏切除同时尾静脉注射阿霉素的方法建立肾小球硬化大鼠模型,SD大鼠随机分为空白对照组、模型组、参地补肾胶囊组、贝那普利组、苏黄泄浊丸组,检测血肌酐( Scr)、尿素氮( BUN)、血浆蛋白( Alb)；免疫组化法检测MMP-9、TIMP-1的表达,计算MMP-9/TIMP-1比值,RT-PCR检测受体TIMP-1mRNA的表达水平。结果：与模型组比较,参地补肾胶囊组能抑制TIMP-1及其受体mRNA的表达(P<0.01),上调MMP-9表达(P<0.01),提高MMP-9/TIMP-1的比值。结论：参地补肾胶囊可抑制TIMP-1及其受体mRNA的表达,改善肾脏病理形态,进而延缓肾小球硬化进展。