Objective; To investigate the possible mechanism of Gantai capsule on resisting hepatic fibrosis induced by alcohol in rats. Methods: Forty rats were randomly divided into control group, model group and Cantai-treated prophylaxis group, light and electron microscope were adopted to observe the histopathology of the hepatic tissue. Masson dyeing and PC-Ⅲ, HA, Ⅳ-C in the serum were evaluated to reflect the level of hepatic fibrosis. Meanwhile, blood, lipids, liver MDA, SOD, and GSH were measured and then used immunohistochemistry to measure the expression of a-SMA in liver tissue. Results: Compared with model group, it revealed that Cantai-treated prophylaxis group rats: ① The degree of fibrosis was improved. ② Liver collagen fibers markedly was decreased. ③ Serum hyaluronic acid and laminin obviously was decreased. ④ liver function was improved. Conclusion: Gantai capsule has an effect on alcohol liver disease and can significantly alleviate the alcohol-induced hepatic fibrosis injury in rats.%目的:探讨肝泰胶囊对大鼠酒精性肝病(ALD)的防治作用及其可能的机制.方法:将40只SD雄性大鼠随机分为正常组10只,采用普通饲料喂养；模型组18只,高脂饲料喂养,同时灌喂酒精性混合液；预防组12只,高脂饲料喂养,同时给予肝泰胶囊和酒精混合液.实验时间为4个月.采用光镜、电镜观察大鼠肝组织病理学改变；Masson染色观察各组大鼠肝纤维化程度；采用放射免疫法测定血清透明质酸(HA)、Ⅲ型前胶原(PC-Ⅲ)、Ⅳ型胶原(Ⅳ)的含量；用半自动生化仪检测丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)；分光光度仪检测丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、y-谷氨酰转肽酶(γ-GT)；免疫组化检测α-平滑肌蛋白(α-SMA)抗体的表达.结果:预防组与模型组比:①预防组大鼠肝组织肝纤维化程度明显改善；②肝组织胶原纤维面积明显减少；③血清透明质酸显著降低；④肝功能损伤明显减轻.结论:肝泰胶囊能显著改善酒精性肝病大鼠的肝损伤,提示该药对酒精性肝病有一定的防治作用.
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