苦杏仁苷对二甲基亚硝胺诱导的大鼠肝纤维化的防治作用

摘要

Objective: To study the prevention effects of amygdalin on rat fibrosis induced by DMN. Methods: Fibrosis model in rats was duplicated with DMN. After being injected with DMN for 4 weeks, model rats were randomly divided into model group, amygdalin treated group and colchicine treated group. Samples were harvested at the end of 6th week after being given corresponding drugs for 2 weeks. The fallowings were detected: yi5The content of liver Hydroxyproline ( Hyp); ②The liver pathology (Sinus red staining and HE staining) ; ③The activity of serum alanine aminotransferase (ALT) , aspartate aminotrans-ferase (AST) , γ-glutamyltransferase (-y-GT) and the content of albumin (Alb) , total bilirubin (Tbil) in serum; ④The activity of liver superoxide dismutase ( SOD) , Glutathione (GSH) , glutathione peroxidase ( GSH-Px) and the content of liver ma-leic dialdehyde (MDA) . Results; ①The content of liver Hyp increased significantly in model group , but it significantly decreased in amygdalin treated group and colchicine treated group; ②There were serious fibrosis and inflammation in model group.Amygdalin and colchicine can obviously alleviate fibrosis and inflammation; ③Activity of serum ALT, AST , γ-GT and content of serum Tbil of the model group significantly increased compared with the normal group; ATL and AST decreased significantly in amygdalin treated group and colchicine treated group; ④There were significant increases in MDA and significantly decrease in SOD, GSH and GSH-Px. Amygdalin could obviously increase GSH. Colchicine could obviously increase GSH and GSH-Px. Conclusion; Amygdalin has good experimental prevention efficacy on rat fibrosis induced by DMN.%目的:探讨苦杏仁苷对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化的防治作用.方法:采用Wistar雄性大鼠,以腹腔注射DMN诱导大鼠肝纤维化模型.在造模4周后,将造模大鼠按体重分层随机分为模型组、苦杏仁苷组及对照药秋水仙碱组,每组10只,灌胃用药两周后取材.观察检测以下指标:①末次体重、肝脾比值；②肝组织羟脯氨酸含量；③肝功能指标:血清丙氨酸氨基转移酶( AHT)、门冬氨酸氨基转移酶(AST)、谷胺酰转肤酶(GGT)活性；白蛋白(Alb)、总胆红素(TBil)含量；④肝组织天狼星红染色及HE染色；⑤肝组织脂质过氧化指标:超氧化物歧化酶( SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肤过氧化物酶(GSH-Px).结果:①模型组大鼠肝组织羟脯氨酸含量与正常组相比显著升高(P＜0.01)；与模型组相比,苦杏仁苷及秋水仙碱治疗组大鼠肝组织羟脯氨酸含量显著降低(P＜0.01)；②天狼星红染色显示模型组大鼠窦周胶原沉积明显,肝小叶问形成较厚汇管区和中央静脉问的纤维间隔,可见较多完整的假小叶,HE染色结果显示模型组大鼠肝细胞水肿明显,汇管区结缔组织有不同程度增生,肝组织中有炎性细胞浸润,窦壁细胞增生明显；苦杏仁苷治疗组和秋水仙碱对照组与模型组相比炎症缓解、胶原纤维增生程度分期显著降低(P＜0.05)；③模型组大鼠血清ALT、AST、GGT活性及TBil含量显著升高,Alb含量显著降低(P＜0.01)；苦杏仁苷组与秋水仙碱组大鼠血清ALT、AST活性显著降低(P ＜0.01或P＜0.05)；④与正常组比较,模型组大鼠肝组织SOD活性、GSH含量及GSH-Px活性显著降低,MDA含量显著升高(P＜0.05)；苦杏仁苷能显著升高模型大鼠肝组织GSH活性(P＜0.05),秋水仙碱则能显著升高GSH、GSH-Px活性.结论:苦杏仁苷对DMN诱导的大鼠肝纤维化有显著改善作用.