益脾养肝方对大鼠肝癌前病变病理形态和脾脏T细胞亚群的影响

摘要

Objective:To observe the effect of compound yipi yanggan Fang(YPYGF)on liver precancerous lesion and T cell subsets of spleen in rats.Methods:Totally 80 rats were randomly divided into the normal control group,the DENa group,and two YPYGF(0.5 and 1. 0 g/ml)treatment groups.After 16 weeks of administration,rats were sacrificed to messure the changes of liver function,liver tissue and T cell subpopulations in rat spleen sections.Results: Compared with the DEN group, the serum levels of ALT,AST,ALP in YPYGF groups were significantly lower,the effect of high -dose group was better(P<0.01).HE and Masson staining pathological sections showed that compound yipi yanggan Fang could improve the pathological changes and change the expression of T cell subsets in spleen in the process of liver precancerous lesions.Conclusion:Compound yipi yanggan Fang could mediate immune function of organism to inhibit the progression of liver precancerous lesions in rats.%目的:观察益脾养肝方对二乙基亚硝胺(DEN)诱导的大鼠肝癌癌前病变病理形态和脾脏T细胞亚群的影响.方法:将80只大鼠随机分为空白组、模型组、益脾养肝方大、小剂量组,腹腔注射DEN制备肝癌癌前病变模型,16周后处死大鼠,检测肝功能、肝组织病理变化,脾脏组织免疫荧光染色观察CD3+、CD4 +和CD8 +T细胞表达情况.结果:与模型组比较,益脾养肝方小、大剂量均能显著降低ALT、AST、ALP水平,且益脾养肝方大剂量组疗效更好(P<0.01);肝组织HE、Masson染色病理切片显示,益脾养肝方大、小剂量组大鼠肝癌前病变病理形态明显改善;脾脏组织免疫荧光染色结果显示,益脾养肝方可提高CD3+、CD4 +T细胞数量,降低CD8+T细胞数量.结论:益脾养肝方对大鼠肝癌前病变进展具有抑制作用,其作用机理可能是通过调节机体的免疫功能抑制肝癌形成.