Objective To investigate the expression of NO, NOS, SOD and MDA, and the effect of Zhiwei Fangbian Capsule on them in chronic atrophic gastritis rats with qi-deficiency and blood-statsis. Methods Ninty rats were divided into 6 groups randomly: normal group, model group, Weimeisu group and Zhiwei Fangbian high, middle, low dose group. Model of chronic atrophic gastritis was induced by MNNG and ranitidine hydrochloride, combined with irregular diet. The effects of Zhiwei Fangbian Capsule on the levels of NO, NOS, SOD and MDA in gastric tissues were detected. Results In model group, levels of NO and NOS in gastric tissues were much higher than those of normal group (P<0.01, P<0.05), level of SOD was much lower than that of normal group (P<0.01), and level of MDA was much higher than that of normal group (P<0.01). Compared with model group, Zhiwei Fangbian Capsule could decrease the content of NO, NOS and MDA in gastric tissues (P<0.05), and increase the level of SOD (P<0.05). Conclusion Zhiwei Fangbian Capsule has functions of ameliorating gastric mucosal blood flow and microcirculation, elevating antioxidation enzyme activity and lessening free radical impairment.%目的 观察治萎防变胶囊对气虚血瘀型慢性萎缩性胃炎(CAG)大鼠胃黏膜一氧化氮(N0)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)及丙二醛(MDA)表达的影响,探讨其作用机制.方法 将90只大鼠随机分为空白组、模型组、维酶素组及治萎防变胶囊大、中、小剂量组,采用综合法即N-甲基-N-硝基-N-亚硝基胍(MNNG)合并盐酸雷尼替丁以及饥饱失常喂养法成功复制CAG动物模型,并应用治萎防变胶囊和维酶素治疗后,分别对各组大鼠胃黏膜组织NO、NOS、SOD及MDA含量进行检测.结果 与空白组比较,模型组大鼠胃黏膜组织NO、NOS水平显著升高(P＜0.01,P＜0.05),SOD活性显著下降(P＜0.01),MDA含量显著升高(P＜0.01)；与模型组比较,治萎防变胶囊可显著降低胃黏膜组织NO、NOS(P＜0.05),显著提高SOD活性(P＜0.05),显著降低MDA含量(P＜0.05),且以治萎防变胶囊大剂量组作用明显.结论 治萎防变胶囊具有提高机体抗氧化能力、减轻自由基损伤的作用.
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