傅立叶变换红外光谱法鉴别金银花挥发油

摘要

Objective To set up a method for identification of Lonicerae japonicae flos volatile oils using Fourier-transform infrared spectroscopy. Methods The volatile oils of Lonicerae japonicae flos and Lonicerae flos was extracted by steam distillation combined with continuous liquid-liquid extraction with hexane. An oil film was prepared for Fourier-transform infrared spectroscopy scanning by dropping the volatile oils solution on the KBr disc and evaporating the solvent. The obtained infrared spectrum was treated by baseline removing and median filter smoothing. The spectral data within 1800-850 cm-1 was selected as the characteristic spectrum for hierarchical cluster analysis. And the volatile oils of Lonicerae japonicae flos and Lonicerae flos were discriminated by the result of hierarchical cluster analysis. Results Enough volatile oils were extracted for obtaining Fourier-transform infrared spectrum from small amount of Lonicerae japonicae flos. The method developed in the study was able to discriminate Lonicerae japonicae flos volatile oils from Lonicerae flos volatile oils. Conclusion The method can be used for identification of Lonicerae japonicae flos volatile oils.%目的研究应用傅立叶变换红外光谱鉴别金银花挥发油的方法。方法用水蒸气蒸馏结合正己烷连续液-液萃取法提取金银花、山银花的挥发油；将挥发油溶液滴在溴化钾窗片上，待溶剂挥发后形成油膜，用傅立叶变换红外光谱法测定红外光谱；将红外光谱进行基线趋势去除和曲线平滑处理；选取1800～850 cm-1范围内光谱数据作为特征光谱进行聚类分析，根据聚类分析结果区分金银花和山银花挥发油样品。结果从少量金银花药材中提取到挥发油用于获取傅立叶变换红外光谱，所采用的数据分析方法能区分金银花和山银花挥发油。结论本方法可以用于鉴别金银花挥发油。