不同工艺提取的竹节参皂苷粗提物毒性及其抗炎作用初步研究

摘要

Objective To compare the toxicity and anti-inflammatory effect of total saponins from Panax japonicus by different extraction technology. Methods The total saponins of sample 1, sample 2, sample 3, sample 4, sample 5 and sample 6 was prepared respectively by different process, and RAW264.7 cells were treated with the samples of different concentration. Then cells morphology was observed under microscope, thiazolyl blue (MTT) method was used to detect cell activity, the nitric oxide (NO) release of RAW264.7 cells was detected with NO kit. Results The cell toxicity of different samples from low to high was as follows:sample 4sample 5>sample 2>sample 6>sample 1>sample 3. Conclusion Among these six different kinds of extraction process of total saponins from Panax japonicus, the total saponins extract by foam fractionation has not only the minimal toxicity, but also the best primary anti-inflammatory effect.%目的：比较不同工艺提取的竹节参皂苷粗提物的毒性及抗炎效果。方法采用不同工艺分别制备竹节参皂苷粗提物样品1、样品2、样品3、样品4、样品5、样品6,然后用不同工艺、不同浓度的样品处理RAW264.7细胞,显微镜下观察细胞形态,噻唑蓝(MTT)法检测细胞活力,一氧化氮(NO)试剂盒检测 RAW264.7细胞 NO 释放量。结果不同样品作用于RAW264.7细胞毒性从小到大依次为：样品4＜样品6＜样品5＜样品2＜样品1＜样品3。不同样品对脂多糖刺激的RAW264.7细胞NO释放抑制效果依次为：样品4＞样品5＞样品2＞样品6＞样品1＞样品3。结论6种不同提取工艺得到的竹节参皂苷粗提物中,泡沫分离法提取的竹节参皂苷粗提物毒性最小,抗炎效果最好。