天芪益智颗粒对阿尔茨海默病大鼠脑组织线粒体及能量代谢的影响

摘要

Objective To investigate the action mechanism of Tianqi Yizhi Granules for Alzheimer disease. Methods Ninety SD rats were divided into sham-operation group, model group, positive medicine group, high, medium and low dosage of Tianqi Yizhi Granules groups. SD rats were injected with aggregated amyloid β1-42 protein (Aβ1-42) into their left ventricle to establish AD models. Treatment groups received gavage with Huperzine A or Tianqi Yizhi Granules. The sham-operation group and model group received gavage with the same volume of normal saline. The mitochondrial membrane potential, activity of complex Ⅰ, Ⅱ, Ⅲ and Ⅳ of mitochondrial respiratory chain, enzymatic activity of Na+-K+ATP, energy charge, and space leaning ability were detected. Results Compared with model group, Tianqi Yizhi Granules could significantly improve mitochondrial membrane potential, activity of complex Ⅰ, Ⅲ and Ⅳ of mitochondrial respiratory chain, enzymatic activity of Na+-K+ATP, space leaning ability, and energy metabolism of brain in AD rats. Conclusion Tianqi Yizhi Granules showed mitochondrial protective capacity, and could improve energy metabolism of brain in AD rats, and then improve AD rats’ space learning ability.%目的：探讨天芪益智颗粒治疗阿尔茨海默病(AD)的作用机制。方法将SD雄性大鼠随机分为假手术组、模型组、阳性药组及天芪益智颗粒高、中、低剂量组,大鼠左侧脑室注射聚集态Aβ1-42建立AD模型,各治疗组给予相应药物灌胃,假手术组和模型组大鼠给予等体积生理盐水,每日1次,连续30 d。检测大鼠脑组织线粒体膜电位、线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ活性,Na+-K+ATP酶活性,脑组织能荷及空间学习记忆能力。结果与模型组比较,天芪益智颗粒可显著增加AD大鼠脑组织线粒体膜电位,提高呼吸链复合物Ⅰ、Ⅲ、Ⅳ、Na+-K+ ATP酶活性和AD大鼠脑能量代谢水平,改善AD大鼠空间学习记忆能力。结论天芪益智颗粒具有线粒体保护作用,可改善AD大鼠脑组织能量代谢,进而提高其空间学习记忆能力。