多指标综合评分法优选三拗汤提取工艺研究

摘要

Objective To simultaneously determinate the contents of four active ingredients (Ephedrine Hydrochloride, Pseudoephedrine Hydrochloride, Amygdalin and Glycyrrhizinate) ofSan Ao Decoction by HPLC; To optimize the extracting process ofSan Ao Decoction.Methods The orthogonal test was employed. Volume of water, time of extraction and times of extraction were set as investigation factors; the transport rates of Ephedrine Hydrochloride, Pseudoephedrine Hydrochloride, Amygdalin and Glycyrrhizinate and the extraction rates were set as investigation indexes to conduct multi-indexes grading.Results The influence factors of the extraction ofSao Ao Decoction were the times of extraction > decoction time > the volume of water. The best extraction condition was: ten fold water; extract for three times; 1 h for each time.Conclusion The optimized extraction process is stable and feasible.%目的：采用高效液相色谱法同时测定三拗汤提取液中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷和甘草酸4种有效成分的含量，优选三拗汤最佳提取工艺条件。方法采用正交试验，以加水量、煎煮时间和煎煮次数为考察因素，以盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷和甘草酸转移率以及出膏率为考察指标，进行多指标综合评分。结果三拗汤提取影响因素依次为煎煮次数＞煎煮时间＞加水量。最佳提取工艺条件为加10倍量水，煎煮3次，每次1 h。结论本研究优选得到的提取工艺稳定可行。