目的：观察青藤碱关节腔注射对兔膝骨关节炎模型软骨及滑膜血管内皮生长因子（VEGF）和神经生长因子（NGF）表达的影响，探讨其相关机制。方法采用木瓜蛋白酶关节腔注射法诱导膝骨关节炎模型。40只新西兰大白兔随机分为空白组、模型组、透明质酸钠组和青藤碱组，空白组不予任何处理，模型组、透明质酸钠组和青藤碱组分别给予生理盐水、玻璃酸钠注射液和正清风痛宁注射液干预30 d，免疫组化检测兔关节软骨及滑膜 VEGF、NGF 的表达。结果与空白组比较，模型组软骨及滑膜 VEGF、NGF 表达显著升高（P＜0.01）；与模型组比较，青藤碱组软骨及滑膜 VEGF、NGF 表达显著降低（P＜0.05，P＜0.01）；青藤碱组软骨及滑膜VEGF、NGF 表达低于透明质酸钠组（P＜0.05，P＜0.01）。结论青藤碱膝关节腔注射可下调 VEGF 和 NGF在膝骨关节炎兔关节软骨及滑膜的表达。%Objective To observe the effects of intra-articular injection of sinomenine on the expressions of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) in articular cartilage and synovium of rabbit knee osteoarthritis models; To discuss its mechanism of action. Methods Totally 40 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by using intra-articular injection of papain enzyme and were treated with saline, sodium hyaluronate and sinomenine respectively for 30 d, except for the control group. After treatment, method of immunohistochemistry was used to detect the expressions of VEGF and NGF in rabbit articular cartilage and synovium. Results Compared with the control group, the expressions of VEGF and NGF in articular cartilage and synovium of model group significantly increased (P<0.01); compared with the model group, the expressions of VEGF and NGF in articular cartilage and synovium of sinomenine group significantly decreased (P<0.05, P<0.01); the expressions of VEGF and NGF in articular cartilage and synovium in sinomenine group were lower than hyaluronate group (P<0.05, P<0.01). Conclusion Intra-articular injection of sinomenine can down-regulate the expressions of VEGF and NGF in articular cartilage and synovium of rabbit knee osteoarthritis.
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