阿奇霉素上调生物被膜阳性铜绿假单胞菌Ⅰ类整合酶基因的表达

摘要

Objective To evaluate the effect of azithromycin on class Ⅰ integron-integrase gene (intI 1 )mRNA expression in biofilm-forming (BF)Pseudomonas aeruginosa (PA).Methods intI 1 of 10 PA strains isolated from a hospital were detected,1 strain with positive BF+intI 1 was selected for culture,blank control group and three az-ithromycin trial groups (divided according to 3 concentrations:16 mg/L,32 mg/L,and 64 mg/L)were set,experi-ments were repeated 5 times,expression of intI 1 mRNA were detected by RT-PCR.Results Relative expression of intI 1 mRNA in azithromycin groups of 16 mg/L,32 mg/L,64 mg/L,and control group were (1 .15 ±0.04), (12.47±3.10),(19.71 ±0.78 ),and (1 .00 ±0.00),respectively,there were significant difference among four groups(F =163.82,P <0.001 );intI 1 mRNA expression between 16mg/L azithromycin group and control group was not significantly different (P >0.05),but among other groups were significantly different (P <0.05 ),intI 1 mRNA expression in azithromycin groups increased with the enhancing concentration of azithromycin in culture so-lution .Conclusion Expression of intI 1 gene mRNA in BF PA can be up-regulated by the present of azithromycin, which may improve the probability of drug-resistant genes,and promote drug-resistant gene recombination.%目的：探讨阿奇霉素对生物被膜（BF）阳性铜绿假单胞菌（PA）Ⅰ类整合酶基因（intI 1）mRNA 表达量的影响。方法对某院临床分离的10株 PA 进行 intI 1基因检测，选择其中1株 BF＋intI 1基因阳性的 PA 进行培养，并设空白对照组和阿奇霉素处理组（按阿奇霉素浓度不同分为3个浓度组，分别为16、32、64 mg／L 组），重复试验5次，采用荧光定量聚合酶链式反应（RT－PCR）检测其 intI 1 mRNA 的表达情况。结果16 mg／L阿奇霉素组、32 mg／L阿奇霉素组、64 mg／L 阿奇霉素组和对照组 intI 1 mRNA 相对表达量分别为（1．15±0．04）、（12．47±3．10）、（19．71±0．78）和（1．00±0．00），各组间比较，差异有统计学意义（F ＝163．82，P ＜0．001）；组间两两比较，除低浓度阿奇霉素组（16 mg／L）与对照组比较，差异无统计学意义（P ＞0．05）外，其他各组间比较，差异均有统计学意义（P ＜0．05），阿奇霉素组 intI 1 mRNA 表达量随培养液中阿奇霉素浓度升高而升高。结论在阿奇霉素压力下 BF 阳性的 PA，intI 1表达有所上调，可提高耐药基因的捕获概率，促进耐药基因重组。