重复性注射镉金属硫蛋白诱导小鼠肾脏rn金属硫蛋白基因的表达

摘要

Objective　To explore the changes of metallo thionein(MT) gene expression induced by multiple short-interval injections of cadmium-metalloth ionein(CdMT) in mice. Methods　With semi-quantitative RT -PCR,gene expression of MT1 and MT2 in renal cortex were studied following an initial CdMT dose and four subsequent doses administered subcutaneously at an interval of 2 hrs. Results　MT1 and MT2 gene expression i n renal cortex increased with the increasing of CdMT dose.At the dose of 0.60 m g/kg,MT1 and MT2 gene expression in renal cortex(1.60±0.12 and 1.44±0.05,respe ctively) were significantly higher than those of controls(1.22±0.1 6 and 0.92±0.12,P<0.05,P<0.01 respectively).MT1 and MT2 gene ex pression in renal cortex were correlated to Cd level in renal cortex(r=0.72 and r=0 .71 respectively，P<0.01). Conclusion　Both MT1 and MT 2 gene expression in renal cortex could be obviously induced by cadmium.There w ere similar changing tendencies between MT1 and MT2 gene expression in renal cor tex.%目的　探讨重复性注射镉金属硫蛋白(CdM T)对小鼠肾脏金属硫蛋白(MT)基因表达的影响。方法　以β-actin 为内参照，采用半定量RT-PCR方法检测重复性CdMT染毒后小鼠肾脏MT1和MT2基因的表达水 平及与染镉剂量和肾皮质镉含量之间的相关性。结果　随着CdMT剂 量的增加，MT1、MT2基因的表达水平都相应升高，当CdMT染毒总剂量达到0.60 mg/kg时，肾 皮质中 MT1、MT2基因的表达水平分别为1.60±0.12及1.44±0.05，与相应的对照组(1.22 ±0.16及0.92±0.12)相比,均明显升高,差异有显著性(P<0.05,P<0.01) ；肾皮质中MT1及MT2基因表达水平分别与肾皮质中镉的含量呈正相关，其相关系数分别为 r=0.72(P<0.01)及r=0.71(P<0.01)。结论　镉对MT1及MT2基因表达具有较强的诱导能力；肾皮质中MT1和 MT2基因表达的变化趋势基本相同