噪声暴露水平对铜锌超氧化物歧化酶基因单核苷酸多态性与噪声性听力损失易感性的影响

摘要

目的 探讨噪声暴露强度和累积噪声暴露量(cumulative noise exposure,CNE)对铜锌超氧化物歧化酶基因(SOD1)rs2070424,rs10432782单核苷酸多态性(single nucleotide polymorphisms,SNPs)与噪声性听力损失(noise induced hearing loss,NIHL)易感性关系的影响.方法 利用病例对照研究,通过比较同一噪声暴露强度噪声作业人员的左耳3000 Hz频段听阈位移情况,筛选出听阈位移最大的10％个体作为本研究的易感人群组,共201例,听阈位移最小的10％个体作为耐受人群组,共202例,并进行相关的职业卫生调查和问卷调查.噪声暴露强度的检测根据GBZ/T 189.8-2007《工作场所物理因素测量-噪声》,抽取易感人群和耐受人群空腹外周静脉血5 ml用于常规方法抽提基因组DNA,TaqMan探针法化学荧光等位基因鉴别试验检测SNP.结果 在噪声暴露强度85～92 dB(A)组内,rs2070424 AA基因型与GG基因型相比,NIHL的患病风险较低,OR值为0.37(95％CI:0.17～0.80).CNE＞82 dB(A)组内,rs2070424 AA基因型是NIHL的保护因素,与GG基因型相比,OR值为0.25(95％CI0.09～0.70).噪声暴露强度85～92 dB(A)组携带rs10432782 GG基因型患NIHL的风险与TT基因型相比OR值为3.17(95％CI:1.16～6.89).GT基因型与TT基因型相比OR值为2.39( 95％CI:1.16～4.97).CNE 75～82 dB(A)组内,携带GG基因型患NIHL的风险与TT基因型相比,OR值为2.35 (95％CI:0.96～5.72),P=0.06,GG基因型可能是NIHL的危险因素.结论 噪声暴露强度和累计噪声暴露量对SOD1rs2070424、rs10432782 SNPs与NIHL.易感性关联存在影响.%Objective To explore the effects of noise exposure level and cumulative noise exposure (CNE) on the relationship between rs2070424 and rs10432782 SNPs in SOD1 and the susceptibility to noiseinduced hearing loss (NIHL).Methods A case-control study was performed for investigating the effects of environmental risk factors on the susceptibility to NIHL in 201sensitive workers and 202 resistant workers.A questionnaire was utilized to investigate the occupational health and to identify the occupational risk factors.The noise exposure levels were detected according to the Chinese standard Measurement of noise in the workplace (GBZ/T 189.8-2007).The peripheral blood samples (5 ml blood for each sample) were from sensitive workers and resistant workers.Genomic DNA was extracted on the basis of the standard procedures of Takara kit.SNPs were detected using standard procedures of TaqMan probe allele identification method.Results In group exposed to 85-92 dB noise (A),the risk of NIHL in the subjects with the AA genotype of rs2070424 was lower than that in the subjects with the GG genotype,OR＝0.37 (95％CI:0.17～0.80).In group exposed to ＞ 82dB CNE (A),the AA genotype of rs2070424 is a protective factor of NIHL,as compared with the GG genotype,OR=0.25 (95％CI:0.09～0.70).In group exposed to 85-92 dB noise (A),the risk of NIHL in the subjects with the GG genotype of rs 10432782 was compared with the risk of NIHL in the subjects with the TT genotype,OR=3.17 (95％CI:1.16～6.89).The GT genotype was compared with TT genotype,OR=2.39(95％ CI:1.16～4.97).In group exposed to 75～82 dB CNE (A),the risk of NIHL in the subjects with the GG genotype was compared with the risk of NIHL in the subjects with the TT genotype,OR=2.35 (95％CI:0.96～5.72),P=0.06.The GG genotype may be a risk factor of NIHJ.Conclusion The noise exposure level and CNE may influence the relationship between rs2070424,rs10432782 SNPs in SOD 1 and noise-induced hearing loss.