Objective To investigate the prevalence of hypertension in ocean seamen and major influencing factors,as well as the association between hypertension and serum microRNA21 and microRNA133a.Methods Health examination and a questionnaire survey were performed for 780 ocean seamen who underwent physical examination in an international travel healthcare center in Fujian,China from January to June,2014.TaqMan RT-qPCR was used to measure the serum levels of microRNA21 and microRNA133a in seamen with hypertension.Results The prevalence of hypertension differed significantly between the ocean seamen with different ages,education levels,marital status,body mass index (BMI) values,drinking frequencies,and numbers of sailing years (P＜0.05).The prevalence rate of hypertension in the ocean seamen increased with the increasing drinking frequency (x2=9.02,P＜0.05),decreased with the increase in degree of education (x2=1 1.578,P＜0.05),and increased with the increase in the number of sailing years (x2=28.06,P＜0.05).The hypertensive ocean seamen had significantly higher expression levels of microRNA21 and MicroRNA133a than the healthy ocean seamen (microRNA21:7.87±5.46 vs 1.03 ±0.80,P ＜0.05;MicroRNA133a:7.45±1.94 vs 4.52±1.15,P＜0.05).The multivariate analysis showed that a high level of microRNA21 (OR=1.61,95％CI:1.22～2.11),a high level of microRNA133a(OR=1.52,95％CI:1.24～1.87),drinking(OR=1.64,95％CI:1.08～2.50),overweight based on BMI(OR=1.18,95％CI:1.07～1.30),and many sailing years (OR=2.89,95％CI:1.14～7.30) were risk factors for hypertension.Conclusion The prevention and treatment of hypertension in ocean seamen should be enhanced.Excessive drinking should be controlled,and sailing time should be arranged reasonably.The microRNA21 and microRNA133a may be associated with the development and progression of hypertension in ocean seamen.%目的 了解远洋船员高血压的流行现状及其主要影响因素,初步探讨高血压与血清microRNA21、microRNA133a的关系.方法 于2014年1至6月,选取在福建某国际旅行卫生保健中心进行体检的780名远洋船员进行健康检查及问卷调查;采用Taqman实时荧光定量聚合酶链式反应(RT-qPCR)技术测定高血压船员血清microRNA21、microRNA33a表达水平.结果 不同年龄、学历、婚姻状况、体重指数(BMI)、饮酒频率及航海年限远洋船员高血压患病率不同,差异均有统计学意义(P＜0.05);远洋船员高血压患病率随饮酒频率的增加而增加(x2=9.02,P＜0.05),随文化程度增高而降低(x2=11.578,P＜0.05),随航海年限增加而增加(x2=28.06,P＜0.05);远洋船员中高血压组microRNA21表达水平(7.87±5.46)高于健康组(1.03±0.80),差异有统计学意义(P＜0.05);MicroRNA133a表达水平(7.45±1.94)高于健康组(4.52±1.15),差异有统计学意义(P＜0.05).多因素分析结果显示,microRNA21(OR=1.61,95％CI=1.22～2.11)与microRNA 133a(OR=1.52,95％CI=1.24～1.87)水平偏高、饮酒(OR=1.64,95％CI =1.08～2.50)、体质指数超重(OR=1.18,95％CI=1.07～1.30)、航海年限较长(OR=2.89,95％CI=1.14～7.30)是高血压的危险因素.结论 应加强远洋船员高血压的防治工作,控制过量饮酒,合理安排航海时间;microRNA21与microRNA133a可能与远洋船员高血压的发生发展存在关联性.
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