慢性乙型肝炎患者外周血CD8+T细胞TCR Vβ基因亚家族克隆化的研究

摘要

目的:分析慢性乙型肝炎(CHB)患者CD8+T细胞TCR Vβ基因亚家族克隆化特征.方法:采用逆转录-聚合酶链反应(RT-PCR)扩增8例CHB患者外周血CD8+T细胞TCR Vβ基因22个亚家族的 CDR3区,基因扫描技术对TCR Vβ亚家族的克隆化进行鉴定.结果:基因扫描显示所有8例CHB 患者CD8+T细胞TCR Vβ基因亚家族均出现一个或一个以上单克隆或寡克隆增生.Vβ8、Vβ11、Vβ12 出现单克隆增生的频率相对较高.8例健康者TCR Vβ基因亚家族均为多克隆.结论:CHB患者外周血CD8+T细胞TCR Vβ亚家族存在克隆性增生.%0bjective :To analyze cbnality of TCR Vβ gene subian flies on perpheralbbod CD 8+ T lymphocyte in patients with chronic hepatitisB.Methods :Exptression of the 22 TCR V(3 gene subian ilieswas analyzed using reverse transcription-polym erase chain reaction (RT- PCR ) ,which am plified the can plem entariy detem ining region 3 (CD R3 ) of TCR Vβ gene on peripheral blood CD 8 T lym phocytes from 8 caseswilh chronic hepatitis B.PCR producte were further analyzed by genescan to identify TCR V βsubfam flies cbnality.Results :Some TCR Vβ gene subian flies showed monoclonalor oligocbnal expansion in the 8 CHB patients.V β8 ,V β11 ,Vβ12 had higher frequency of monocbnal expansion.All TCR Vβ gene fam flies of CD 8+ T imphocyte showed a Gaussian distribution in nomal control cases.Conclusion :TCR V(3 subian flies on CD8+T lymphocyte of CHB patients during acute exacerbation showed clonal expansion.