促红细胞生成素对荷瘤小鼠免疫功能的影响

摘要

目的:检测促红细胞生成素(Erythropoietin,EPO)对荷黑色素瘤小鼠免疫功能的影响.方法:将C57BL/6小鼠随机分为3组:正常对照组、盐水处理组和EPO处理组,盐水处理组和EPO处理组小鼠皮下接种黑色素瘤细胞B16.17天后,检测各组小鼠外周血中红细胞(RBC)、血红蛋白(Hb)、红细胞压积(Hct)和白细胞(WBC)数量及脾脏脏器指数;应用流式细胞术检测脾细胞CD4+、CD8+T细胞亚群的分布；ELISA检测血清中IL-2和TNF-α细胞因子的水平.结果:盐水处理组荷瘤小鼠外周血中RBC、Hb和Hct含量明显低于正常对照组小鼠(P＜0.05),应用EPO可明显提高荷瘤小鼠外周血中RBC、Hb和Hct含量及脾脏脏器指数(P＜0.05),三组小鼠外周血中WBC数量无明显差别(P＞0.05)；盐水处理组及EPO处理组荷瘤小鼠脾脏CD4+T细胞百分率及CD4+/CD8+T细胞比值明显低于正常小鼠(P＜0.05).EPO处理组与盐水处理组相比,CD4+T细胞百分率无差别(P＞0.05),而CD8+T细胞百分率下降(P＜0.05),致使EPO处理组小鼠CD4 +/CD8+T细胞比值升高(P＜0.05)；盐水处理组及EPO处理组荷瘤小鼠血清中TNF-α、IL-2含量明显低于正常小鼠(P＜0.05),EPO处理组小鼠血清中IL-2水平与盐水处理组相比增高(P＜0.05),但该两组小鼠血清中TNF-α含量无明显差别(P＞0.05).结论:EPO在改善荷瘤小鼠贫血状态的同时,还可通过提高外周血CD4 +/CD8+T细胞比值和IL-2含量而增强小鼠的免疫功能.%Objective; To study the effects of erythropoietin (EPO) on immune function in a murine melanoma model. Methods :C57BL/6 mice were randomly divided into 3 groups; normal control group, saline-treated group and EPO-treated group. Mice of saline-treated group and EPO-treated group were inoculated with melanoma cell B16. The changes of red blood cell( RBC) , hemoglobin ( Hb) , hematocrit ( Hct) and white blood cell( WBC) in peripheral blood, spleen index were observed. The subpopulations of CD4+ and CD8+ T-cell in mice spleen was performed by flow cytomery. Serum concentrations of interleukin (IL) -2 and tumor necrosis factor (TNF)-α of 3 groups was measured by ELISA. Results;A significantly greater reduction in the levels of RBC, Hb and Hct in peripheral blood was observed in saline-treated mice compared to the normal control mice (P < 0. 05) . EPO treatment increased the levels of RBC, Hb and Hct in peripheral blood and spleen index in tumor-bearing mice (P < 0. 05). The levels of WBC in peripheral blood was not different in 3 groups(P > 0. 05); CD4+ T-cell percentage and CD4+ /CD8+ ratio of spleen cell was significantly decreased in tumor-bearing mice compared to the normal control group( P < 0. 05). CD4 + T-cell percentage of mice spleen was not different (P > 0. 05) , but CD8 + T-cell percentage was significantly decreased leading to increase of the CD4 + /CD8 + ratio in EPO-treated group compared to the saline-treated group (P < 0. 05 ) ; the serum concentrations of IL-2 and TNF-α was significantly decreased in tumor-bearing group compared to the normal control group (P < 0. 05 ). The concentration of IL-2 was significantly increased in EPO-treated group compared to the saline-treated group ( P < 0. 05 ) , while that of TNF-α was not different in both study groups ( P > 0. 05 ). Conclusion : These results suggest that EPO treatment can correct the anemia, and also improve immune function in tumor-bearing mice by increasing the CD4+ /CD8 + ratio and IL-2 concentrations of peripheral blood.