姜黄素对佐剂性关节炎大鼠滑膜OPG和RANKL蛋白表达的影响

摘要

Objective:By observing the effects of curcumin on synovial pathology, synovial osteoprotegerin and receptor activator of nuclear factor κB ligand expression factor of adjuvant arthritis rat models, we try to explore the possible mechanism of prevention and treatment of rheumatoid arthritis.Methods: There were three groups, the normal group, the model group and the curcumin group.We made adjuvant arthritis rat models with Freund′s complete adjuvant,and they were divided into the model group and the curcumin group.On d 28 after modeling,all rats were killed.All synovial membranes were sent for routine histological evaluation with HE staining.And synovial RANKL and OPG protein expression were measured by Western blot.Results: Curcumin could influence synovium pathology change of adjuvant arthritis rats,the pathological picture showed that infiltration of joint synovial cell,pro-liferation of synovial fibroblasts in model group rats were significantly increased,compared with normal group( P<0.01).Pathology of rats synovial tissue in rats of curcumin group were significantly improved, compared with that in rats of model group.Expression of RANKL,OPG protein in synovial tissue of curcumin group,based on pairwise comparison between with model group,the results were statistically significant( P<0.01 ).Synovial RANKL/OPG ratio were significantly lower than model group ( P<0.01 ).Conclusion:Curcumin can improve synovial pathology damage of adjuvant arthritis rats,curcumin may exert a protective effect on arthritis rats by regulating OPG/RANKL signaling pathway.%目的：通过观察姜黄素对佐剂性关节炎（ Adjuvant arthritis， AA ）模型大鼠滑膜病理、滑膜骨保护素（ Osteoprotegerin，OPG）、核因子κB受体活化因子配体（ Receptor activator for nuclear factor-κB ligand，RANKL）表达的影响，探讨其防治类风湿关节炎（ Rheumatoid arthritis，RA）可能作用机制。方法：雄性SD大鼠以弗氏完全佐剂造模，分为模型组与姜黄素组，另有正常组，d 28处死所有大鼠行滑膜常规病理HE染色，并用Western blot法来检测滑膜RANKL、OPG蛋白的表达。结果：模型组大鼠滑膜炎性细胞浸润、纤维细胞及滑膜细胞的增生较正常组均明显增加（P＜0.01），而姜黄素组的大鼠滑膜组织病理较模型组显著改善（ P＜0.01）；姜黄素组大鼠的滑膜RANKL表达显著低于模型组，滑膜OPG表达显著高于模型组， RANKL/OPG比值显著低于模型组（ P＜0.01）。结论：姜黄素不仅改善AA大鼠关节滑膜病理学损伤，还可调节滑膜RANKL/OPG的比值。因此姜黄素可能通过调节OPG/RANKL系统来发挥对AA大鼠的治疗作用。