远程缺血后适应中大鼠海马CA1区TLR4和IL-6的表达变化及其意义

摘要

Objective:To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning(RIP) and explore its significance.Methods: All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly.Each group was divided into 4 time points:12h,24 h,48 h and 72 h group.There were 6 rats in each group.Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group.The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min, repeat 3 times.To observe the pathological variation of hippocampus CA1 region by HE dyeing;to test the expression of TLR4 and IL-6 by immunohistochemical staining.Results: Compared to contrast group, neuronal loss and swelling reduced significantly in RIP group.Compared to sham group, the TLR4 and IL-6 expression in contrast group and RIP group increased significantly ( P<0.05 ) .Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly(P<0.05).Conclusion:RIP dose have protective effect on cerebral ischemia.The effect may be associated with the inhibition of TLR4 and IL-6 expression.%目的：：观察大鼠远程缺血后适应时海马CA1区TLR4、IL-6表达变化并探讨其意义。方法：72只雄性SD大鼠随机分为假手术组、对照组、远程缺血后适应组，各组又分为12 h、24 h、48 h、72 h各时间点组，每组6只。应用改良Longa法建立大鼠脑缺血再灌注损伤模型并以此为对照组，在再灌注即刻用止血带结扎／放松双后肢根部各30 min循环3次，作为远程缺血后适应处理方法。 HE染色观察大鼠海马CA1区组织病理学变化，免疫组织化学染色观察大鼠海马CA1区TLR4和IL-6的表达。结果：在大鼠海马CA1区，远程缺血后适应组神经元缺失、细胞肿胀较对照组明显减轻。与假手术组相比，对照组与远程缺血后适应组TLR4和IL-6表达明显增高(P＜0.05）；且远程缺血后适应组TLR4和IL-6的表达较对照组明显减少( P＜0.05）。结论：远程缺血后适应具有脑保护作用，这种作用可能与抑制TLR4和IL-6表达有关。