Genistein联合环孢素 A 对大鼠心脏移植排斥反应中 CXCR3表达的干预

摘要

目的：探讨Genistein联合环孢素A在大鼠同种异体心脏移植急性排斥反应中对CXCR3的影响。方法：近交系Wistar大鼠为供者，SD大鼠为受者，行大鼠颈部心脏移植手术，移植后的大鼠随机分为3组，急性排斥反应( AR）组：心脏移植术后未采取任何治疗；环孢素A( CsA）组：术后接受CsA治疗；CsA+Genistein( C+G）组：术后接受CsA及Genistein的联合治疗。术后7 d，分别采集3组移植心脏，制备成组织切片，HE染色观察移植心脏的病理学改变，免疫组化及Western blot检测CXCR3受体的表达。结果：AR组CXCR3呈强阳性表达，C+G组炎性细胞浸润程度明显减轻，且CXCR3蛋白在心肌组织中呈低表达。结论：Genistein联合环孢素A能明显抑制大鼠心脏移植急性排斥反应中CXCR3的表达，减轻免疫排斥反应。%Objective:To investigate the effect of Genistein combined with cyclosporine A on chemokine receptor CXCR3 in rejection of cardiac allograft in rats.Methods:Heterotopic cervical heart transplantation was performed from Wistar rats to SD rats by using cuff-technique.The SD rat recipients were randomly divided into 3 groups.No treatment was adopted in the acute rejection( AR) group.Rats in the cyclosporine A(CsA) group were treated with cyclosporine A after transplantion.Rats in CsA+Genistein(C+G) group were treated with Genistein combined with cyclosporine A after transplantion.All the cardiac allografts were harvested at 7th day, and made into tissue slices.The HE-staining was used to observe the pathology changes of the allograft myocardia.And the expression of CXCR3 was detected with immunehistochemistry and Western blot.Results:The expression of CXCR3 strongly positively expressed in AR group.The degree of myocardial inflammation in C+G group and the expression of CXCR3 were much lower than the other two groups.Conclusion:Genistein combined with cyclosporine A can significantly relieve rejection of cardiac allograft by inhibiting the ex-pression of CXCR3.